| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FZD4 |
| Uniprot No | Q9ULV1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 37-222aa |
| 氨基酸序列 | ADPFGDEEERRCDPIRISMCQNLGYNVTKMPNLVGHELQTDAELQLTTFT PLIQYGCSSQLQFFLCSVYVPMCTEKINIPIGPCGGMCLSVKRRCEPVLK EFGFAWPESLNCSKFPPQNDHNHMCMEGPGDEEVPLPHKTPIQPGEECHS VGTNSDQYIWVKRSLNCVLKCGYDAGLYSRSAKEFTDIWLEPKSCDKTHT CPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKF NWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSN KALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPS DIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSC SVMHEALHNHYTQKSLSLSPGKHHHHHH |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FZD4重组蛋白的模拟参考文献示例(仅供参考，建议通过学术数据库查询真实文献)：
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1. **文献名称**: *Expression and purification of recombinant human FZD4 extracellular domain for structural studies*
**作者**: Smith A, et al.
**摘要**: 研究报道了人源FZD4胞外结构域的重组表达与纯化方法，利用昆虫细胞表达系统获得高纯度蛋白，并通过X射线晶体学解析其三维结构，揭示了Wnt配体结合的关键位点。
2. **文献名称**: *Functional characterization of FZD4 recombinant protein in Wnt/β-catenin signaling activation*
**作者**: Lee B, et al.
**摘要**: 通过哺乳动物细胞表达重组FZD4蛋白，证实其与Wnt3a配体的结合能力，并证明其在体外模型中激活β-catenin信号通路的活性，为靶向治疗视网膜病变提供依据。
3. **文献名称**: *FZD4 recombinant protein mitigates angiogenesis defects in a mouse model of familial exudative vitreoretinopathy*
**作者**: Chen X, et al.
**摘要**: 利用HEK293细胞表达功能性FZD4重组蛋白，并在FZD4基因突变小鼠模型中验证其通过恢复Wnt信号通路改善异常血管生成的效果，提示潜在治疗应用。
4. **文献名称**: *Development of a FRET-based assay using recombinant FZD4 to screen small molecule modulators*
**作者**: Tanaka K, et al.
**摘要**: 构建基于荧光共振能量转移(FRET)的高通量筛选平台，利用重组FZD4蛋白筛选调控Wnt信号的小分子化合物，发现多个先导分子可调节FZD4与配体相互作用。
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**建议**：可通过PubMed、Google Scholar等平台，以“FZD4 recombinant protein”、“FZD4 expression”、“FZD4 Wnt signaling”等关键词检索最新文献。关注领域内高影响力期刊(如*Nature Communications*、*Journal of Biological Chemistry*等)或疾病相关研究(如视网膜病变、癌症)。
**Background of FZD4 Recombinant Protein**
Frizzled-4 (FZD4) is a member of the Frizzled family of G protein-coupled receptors, which play pivotal roles in the Wnt signaling pathway. This pathway is critical for embryonic development, tissue homeostasis, and stem cell regulation. FZD4 specifically binds Wnt ligands, such as Wnt3a and Norrin, to activate both canonical (β-catenin-dependent) and non-canonical signaling cascades. Dysregulation of FZD4 is implicated in diseases, including cancers, vascular disorders, and familial exudative vitreoretinopathy (FEVR), a rare genetic eye disorder.
Recombinant FZD4 protein is engineered *in vitro* using expression systems (e.g., mammalian cells, *E. coli*) to produce purified, functional FZD4 extracellular domains or full-length proteins. These recombinant variants often retain ligand-binding capability, particularly through their cysteine-rich domain (CRD), enabling studies on receptor-ligand interactions, signaling mechanisms, and therapeutic targeting.
Research applications include:
1. **Mechanistic Studies**: Elucidating Wnt/Norrin-FZD4 signaling in angiogenesis, retinal development, and disease pathways.
2. **Drug Discovery**: Screening for inhibitors/agonists to modulate FZD4 activity in cancers or FEVR.
3. **Structural Biology**: Solving CRD-ligand complex structures (via X-ray crystallography or cryo-EM) to guide rational drug design.
4. **Diagnostic Tools**: Developing assays to detect autoantibodies in autoimmune disorders linked to FZD4 dysfunction.
FZD4 recombinant proteins are also used to generate antibodies for immunohistochemistry, flow cytometry, or functional blocking experiments. Challenges include maintaining post-translational modifications (e.g., glycosylation) critical for ligand binding, necessitating mammalian expression systems for certain applications.
Overall, FZD4 recombinant proteins serve as essential tools for unraveling its biological roles and advancing therapies for Wnt-related pathologies.
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