| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDH7 |
| Uniprot No | Q08722-3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-139aa |
| 氨基酸序列 | QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTF DGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTE LTREGETIIELKYRVVSWFSP |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性举例的参考文献，供参考(请注意，实际文献可能需要通过学术数据库核实)：
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1. **文献名称**: "Recombinant Expression and Functional Characterization of Human Cadherin-7 in Neural Cell Adhesion"
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中表达了重组人CDH7胞外结构域，并验证其钙离子依赖性细胞粘附功能。通过体外结合实验，证实CDH7可能通过同源相互作用参与神经突触形成。
2. **文献名称**: "Structural Insights into CDH7-Mediated Cell-Cell Interaction via Crystallography"
**作者**: Zhang L, et al.
**摘要**: 利用昆虫细胞系统表达并纯化CDH7重组蛋白，通过X射线晶体学解析其EC1-EC5结构域的三维结构，揭示了其独特的钙结合位点及潜在的配体结合界面。
3. **文献名称**: "CDH7 Recombinant Protein as a Biomarker in Colorectal Cancer Progression"
**作者**: Kim S, et al.
**摘要**: 研究通过哺乳动物细胞表达CDH7-Fc融合蛋白，发现其与肿瘤微环境中整合素的相互作用可能促进癌细胞迁移，提示CDH7作为癌症治疗靶点的潜力。
4. **文献名称**: "Optimization of CDH7 Protein Production in HEK293 Cells for Mechanistic Studies"
**作者**: Müller R, et al.
**摘要**: 开发了高效表达CDH7全长重组蛋白的HEK293细胞系，并建立功能性检测方法，为研究其在胚胎发育中的信号传导机制提供工具。
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**注意**：以上内容为模拟生成，实际文献需通过PubMed、Web of Science等平台以关键词“CDH7 recombinant”“Cadherin-7 expression”检索。若研究较少，可扩展至“Cadherin家族重组蛋白”或结合具体功能(如细胞粘附、癌症)筛选。
**Background of CDH7 Recombinant Protein**
CDH7 (Cadherin-7), a member of the cadherin superfamily, is a calcium-dependent cell adhesion molecule implicated in mediating cell-cell interactions and tissue morphogenesis. Classified as a type II classical cadherin, CDH7 plays roles in neuronal development, axon guidance, and synaptic connectivity, with expression observed in specific regions of the central nervous system (CNS) and sensory organs during embryogenesis. Its extracellular domain contains multiple cadherin repeats that facilitate homophilic binding, while the intracellular domain interacts with catenins to link the actin cytoskeleton, enabling mechanical and signaling functions.
The recombinant CDH7 protein is engineered to study its biological activities *in vitro* or *in vivo*. Typically produced in mammalian or insect cell systems to ensure proper post-translational modifications (e.g., glycosylation), the recombinant form retains functional domains critical for adhesion and signaling. Purification tags (e.g., His-tag, Fc-fusion) are often incorporated for isolation and detection.
Research on CDH7 has expanded due to its potential involvement in cancer progression and neurological disorders. In tumors, aberrant CDH7 expression may correlate with metastasis or cell migration, while in neurodevelopmental contexts, dysregulation could impair neural circuit formation. Recombinant CDH7 enables mechanistic studies—such as ligand-receptor interaction assays, cell adhesion/migration assays, or structural analyses—to elucidate its role in health and disease. Additionally, it serves as a tool for developing therapeutic antibodies or biomarkers. Further studies aim to clarify its signaling pathways and therapeutic relevance, positioning CDH7 as a molecule of interest in both basic and translational research.
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