| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | aMSH |
| Uniprot No | O95630 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-424aa |
| 氨基酸序列 | MSDHGDVSLPPEDRVRALSQLGSAVEVNEDIPPRRYFRSGVEIIRMASIY SEEGNIEHAFILYNKYITLFIEKLPKHRDYKSAVIPEKKDTVKKLKEIAF PKAEELKAELLKRYTKEYTEYNEEKKKEAEELARNMAIQQELEKEKQRVA QQKQQQLEQEQFHAFEEMIRNQELEKERLKIVQEFGKVDPGLGGPLVPDL EKPSLDVFPTLTVSSIQPSDCHTTVRPAKPPVVDRSLKPGALSNSESIPT IDGLRHVVVPGRLCPQFLQLASANTARGVETCGILCGKLMRNEFTITHVL IPKQSAGSDYCNTENEEELFLIQDQQGLITLGWIHTHPTQTAFLSSVDLH THCSYQMMLPESVAIVCSPKFQETGFFKLTDHGLEEISSCRQKGFHPHSK DPPLFCSCSHVTVVDRAVTITDLR |
| 预测分子量 | 54 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于aMSH(α-黑素细胞刺激素)重组蛋白的参考文献示例(部分内容可能基于真实研究概括，具体文献需核实)：
1. **《Anti-inflammatory effects of recombinant α-MSH in murine models of endotoxin-induced uveitis》**
- 作者：Rousseau, A. P., et al.
- 摘要：研究通过重组aMSH蛋白在小鼠内毒素诱导的葡萄膜炎模型中验证其抗炎作用，发现其通过抑制NF-κB通路显著减少炎症因子释放。
2. **《Expression and purification of biologically active recombinant α-MSH fusion protein in E. coli》**
- 作者：Zhang, Y., et al.
- 摘要：报道了一种利用大肠杆菌表达系统高效制备重组aMSH的方法，通过亲和层析纯化获得具有体外黑色素生成活性的功能性蛋白。
3. **《Recombinant α-MSH attenuates oxidative stress and skin fibrosis in a murine scleroderma model》**
- 作者：Lee, S. J., et al.
- 摘要：在小鼠硬皮病模型中，重组aMSH通过减少氧化应激和调控TGF-β信号通路，显著改善皮肤纤维化症状。
4. **《Therapeutic potential of α-MSH in melanoma: Recombinant protein delivery inhibits tumor growth》**
- 作者：Slominski, A., et al.
- 摘要：探讨重组aMSH对黑色素瘤的作用，发现其通过激活MC1R受体抑制肿瘤细胞增殖，并增强化疗药物敏感性。
**注**：以上文献信息为示例性质，实际引用时请核对具体来源及准确性。建议通过PubMed、Web of Science等平台检索最新研究。
**Background of Recombinant α-MSH Protein**
α-Melanocyte-stimulating hormone (α-MSH) is a naturally occurring neuropeptide derived from proopiomelanocortin (POMC), playing critical roles in regulating physiological processes such as pigmentation, inflammation, energy homeostasis, and immune response. It binds to melanocortin receptors (MCRs), particularly MC1R, MC3R, MC4R, and MC5R, to exert its effects. Historically, α-MSH’s anti-inflammatory and immunomodulatory properties have drawn attention, as it inhibits pro-inflammatory cytokine release and promotes resolution of inflammation, making it a potential therapeutic candidate for conditions like dermatitis, sepsis, or autoimmune disorders.
Recombinant α-MSH (raMSH) is produced via genetic engineering, often using bacterial (e.g., *E. coli*) or mammalian expression systems to ensure proper post-translational modifications and bioactivity. Unlike synthetic or animal-derived α-MSH, recombinant technology offers scalability, consistency, and reduced risk of contamination. This has facilitated its use in both research and clinical settings.
In dermatology, raMSH is studied for skin repair and pigmentation disorders. Its role in modulating oxidative stress and promoting melanogenesis also supports applications in vitiligo treatment. Beyond dermatology, raMSH shows promise in neuroprotection, obesity management (via appetite regulation), and even as a diagnostic tool in receptor-binding studies. Recent preclinical and clinical trials explore its efficacy in chronic inflammatory diseases, sepsis, and metabolic syndromes.
Despite its potential, challenges remain in optimizing delivery methods (e.g., stability, half-life) and minimizing off-target effects. Ongoing research aims to engineer analogs with enhanced receptor specificity and pharmacokinetic profiles, broadening its therapeutic scope.
In summary, recombinant α-MSH merges natural biology with biotechnological innovation, offering a versatile tool for both understanding melanocortin pathways and developing targeted therapies.
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