| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SATB2 |
| Uniprot No | Q9UPW6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-733aa |
| 氨基酸序列 | MERRSESPCLRDSPDRRSGSPDVKGPPPVKVARLEQNGSPMGARGRPNGA VAKAVGGLMIPVFCVVEQLDGSLEYDNREEHAEFVLVRKDVLFSQLVETA LLALGYSHSSAAQAQGIIKLGRWNPLPLSYVTDAPDATVADMLQDVYHVV TLKIQLQSCSKLEDLPAEQWNHATVRNALKELLKEMNQSTLAKECPLSQS MISSIVNSTYYANVSATKCQEFGRWYKKYKKIKVERVERENLSDYCVLGQ RPMHLPNMNQLASLGKTNEQSPHSQIHHSTPIRNQVPALQPIMSPGLLSP QLSPQLVRQQIAMAHLINQQIAVSRLLAHQHPQAINQQFLNHPPIPRAVK PEPTNSSVEVSPDIYQQVRDELKRASVSQAVFARVAFNRTQGLLSEILRK EEDPRTASQSLLVNLRAMQNFLNLPEVERDRIYQDERERSMNPNVSMVSS ASSSPSSSRTPQAKTSTPTTDLPIKVDGANINITAAIYDEIQQEMKRAKV SQALFAKVAANKSQGWLCELLRWKENPSPENRTLWENLCTIRRFLNLPQH ERDVIYEEESRHHHSERMQHVVQLPPEPVQVLHRQQSQPAKESSPPREEA PPPPPPTEDSCAKKPRSRTKISLEALGILQSFIHDVGLYPDQEAIHTLSA QLDLPKHTIIKFFQNQRYHVKHHGKLKEHLGSAVDVAEYKDEELLTESEE NDSEEGSEEMYKVEAEEENADKSKAAPAEIDQR |
| 预测分子量 | 109 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SATB2重组蛋白的3篇参考文献示例(注：文献为虚拟示例，实际引用需核实)：
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1. **文献名称**: *"Recombinant SATB2 Protein: Purification and DNA Binding Characterization"*
**作者**: Smith A, et al.
**摘要**: 本研究报道了人源SATB2重组蛋白在大肠杆菌中的高效表达及纯化方法，并验证其与核基质结合区(MARs)的特异性相互作用。通过电泳迁移率实验(EMSA)证实重组SATB2能够调控靶基因的染色质结构。
2. **文献名称**: *"Functional Analysis of SATB2 in Osteoblast Differentiation Using Recombinant Protein"*
**作者**: Chen L, et al.
**摘要**: 利用哺乳动物细胞表达系统制备SATB2重组蛋白，研究其在成骨细胞分化中的作用。实验表明，外源性SATB2蛋白可激活碱性磷酸酶(ALP)活性，并促进骨相关基因(如Runx2)的表达。
3. **文献名称**: *"SATB2 Recombinant Protein Attenuates Tumor Growth in Colorectal Cancer Models"*
**作者**: Kim H, et al.
**摘要**: 通过纯化SATB2重组蛋白探究其在结直肠癌中的抑癌机制。体内实验显示，SATB2蛋白通过抑制Wnt/β-catenin通路显著降低肿瘤细胞增殖和转移能力。
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**注**：以上文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Google Scholar)检索真实文献并核对信息准确性。
**Background of SATB2 Recombinant Protein**
SATB2 (Special AT-rich sequence-binding protein 2) is a DNA-binding protein that regulates chromatin organization and gene expression by binding to matrix attachment regions (MARs) in the genome. It functions as a transcriptional regulator, influencing the expression of genes involved in development, differentiation, and cellular identity. SATB2 is particularly critical in embryonic development, where it plays essential roles in craniofacial patterning, osteoblast differentiation, and neuronal development. Mutations or dysregulation of SATB2 are linked to human disorders such as SATB2-associated syndrome (SAS), characterized by intellectual disability, craniofacial anomalies, and speech impairment.
Recombinant SATB2 protein is produced using biotechnological methods, often through expression in bacterial (e.g., *E. coli*) or mammalian cell systems. The recombinant protein retains functional domains, including the CUT domain for DNA binding and a homeodomain for protein interactions. Purification techniques such as affinity chromatography (e.g., His-tag or GST-tag systems) ensure high purity and activity.
In research, SATB2 recombinant protein is widely used to study its role in gene regulation, chromatin remodeling, and signaling pathways. It serves as a tool for *in vitro* DNA-binding assays, protein interaction studies, and functional analyses in cellular models. Additionally, SATB2 is a biomarker in diagnostics; its expression is often assessed in cancers (e.g., colorectal, osteosarcoma) for prognostic or diagnostic purposes.
The development of SATB2 recombinant protein has advanced understanding of its dual roles in development and disease, offering potential therapeutic avenues for genetic syndromes and cancers. Its study continues to uncover mechanisms linking chromatin architecture to cellular function, highlighting SATB2 as a key player in epigenetic regulation.
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