| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Zic |
| Uniprot No | Q15915 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-447aa |
| 氨基酸序列 | MLLDAGPQYP AIGVTTFGAS RHHSAGDVAE RDVGLGINPF ADGMGAFKLN PSSHELASAG QTAFTSQAPG YAAAAALGHH HHPGHVGSYS SAAFNSTRDF LFRNRGFGDA AAAASAQHSL FAASAGGFGG PHGHTDAAGH LLFPGLHEQA AGHASPNVVN GQMRLGFSGD MYPRPEQYGQ VTSPRSEHYA APQLHGYGPM NVNMAAHHGA GAFFRYMRQP IKQELICKWI EPEQLANPKK SCNKTFSTMH ELVTHVTVEH VGGPEQSNHI CFWEECPREG KPFKAKYKLV NHIRVHTGEK PFPCPFPGCG KVFARSENLK IHKRTHTGEK PFKCEFEGCD RRFANSSDRK KHMHVHTSDK PYLCKMCDKS YTHPSSLRKH MKVHESSSQG SQPSPAASSG YESSTPPTIV SPSTDNPTTS SLSPSSSAVH HTAGHSALSS NFNEWYV |
| 预测分子量 | 48,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及Zic重组蛋白研究的参考文献及其摘要概要：
1. **文献名称**：Crystal structure of the zinc-finger domain of the Zic2 protein
**作者**：Hogrebe, A., et al.
**摘要**：报道了Zic2蛋白锌指结构域的晶体结构，揭示了其与DNA结合的分子机制，为重组Zic蛋白的功能研究提供结构基础。
2. **文献名称**：Recombinant Zic1 protein promotes neuronal differentiation of embryonic stem cells
**作者**：Lim, J.W., et al.
**摘要**：研究重组Zic1蛋白在小鼠胚胎干细胞向神经元分化中的作用，证实其通过激活Wnt信号通路调控神经发育。
3. **文献名称**：Expression and purification of functional human Zic3 recombinant protein in E. coli
**作者**：Chen, Y., et al.
**摘要**：开发了在大肠杆菌中高效表达可溶性人源Zic3重组蛋白的方法，并通过体外实验验证其与Gli转录因子的相互作用功能。
注：以上文献信息为示例性内容，实际研究中建议通过PubMed或Web of Science检索具体文献(关键词：Zic protein, recombinant expression, structural analysis, neural development)。
Zic recombinant proteins are engineered versions of the Zic family of zinc finger transcription factors, which play pivotal roles in embryonic development and cellular differentiation. The Zic family (Zic1-5 in mammals) is evolutionarily conserved and shares structural homology with the Drosophila pair-rule gene *odd-paired*. These proteins are characterized by C2H2-type zinc finger domains that mediate DNA binding and interactions with other regulatory molecules. Zic proteins are crucial in early embryogenesis, particularly in neural tube closure, neural crest development, and left-right patterning. Dysregulation of Zic genes is linked to congenital disorders (e.g., holoprosencephaly) and cancers.
Recombinant Zic proteins are typically produced using expression systems like *E. coli* or mammalian cell cultures, followed by purification via affinity tags. Their production enables precise study of Zic protein functions, including their role in maintaining pluripotency in stem cells and reprogramming somatic cells. For example, Zic3 is widely used in combination with other transcription factors to enhance induced pluripotent stem cell (iPSC) generation efficiency. Additionally, recombinant Zic proteins serve as tools to investigate signaling pathways like Wnt and Shh, where Zic proteins act as modulators.
Recent advances in protein engineering have improved the solubility and stability of recombinant Zic proteins, facilitating their application in disease modeling and drug screening. However, challenges remain, such as reproducing post-translational modifications critical for native function. Ongoing research focuses on optimizing expression systems and exploring therapeutic potentials, particularly in regenerative medicine and neurodevelopmental disorder treatments. These recombinant tools continue to bridge gaps between developmental biology and translational applications.
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