| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS6Ka5 |
| Uniprot No | Q04206 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-551aa |
| 氨基酸序列 | MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS |
| 预测分子量 | 60,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPS6KA5重组蛋白的3篇参考文献示例(注：文献为示例性概括，非真实存在)：
1. **文献名称**: "Recombinant Expression and Functional Characterization of Human RPS6KA5 Kinase"
**作者**: Smith J, et al.
**摘要**: 本研究报道了人源RPS6KA5重组蛋白在大肠杆菌系统中的高效表达与纯化方法，并通过体外激酶实验验证了其磷酸化下游底物(如CREB蛋白)的活性，揭示了其在MAPK/ERK信号通路中的调控作用。
2. **文献名称**: "Structural Insights into RPS6KA5 Activation by Phosphorylation"
**作者**: Lee H, et al.
**摘要**: 通过X射线晶体学解析了重组RPS6KA5蛋白的磷酸化激活状态结构，揭示了其N端激酶结构域与C端调控模块的构象变化机制，为设计靶向该激酶的小分子抑制剂提供了结构基础。
3. **文献名称**: "RPS6KA5 Recombinant Protein Attenuates Inflammation in Macrophages via NF-κB Pathway"
**作者**: Chen R, et al.
**摘要**: 研究利用哺乳动物细胞表达系统制备了具有生物活性的重组RPS6KA5蛋白，证明其通过抑制NF-κB信号通路减少炎症因子(如TNF-α、IL-6)的分泌，提示其在炎症性疾病治疗中的潜在应用价值。
(注：以上文献信息为模拟生成，实际研究中请通过PubMed或Web of Science等数据库查询真实文献。)
RPS6KA5 (Ribosomal Protein S6 Kinase A5), also known as MSK1 (Mitogen- and Stress-Activated Protein Kinase 1), is a serine/threonine kinase belonging to the RSK (p90 ribosomal S6 kinase) family. It plays a critical role in cellular signaling pathways, particularly the MAPK/ERK (Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase) cascade, which regulates processes like cell proliferation, survival, differentiation, and stress responses. RPS6KA5 is activated via phosphorylation by upstream kinases such as ERK1/2 or p38 MAPK, enabling it to phosphorylate downstream targets, including transcription factors (e.g., CREB, NF-κB) and histone H3. thereby modulating gene expression and chromatin remodeling.
Recombinant RPS6KA5 protein is engineered for in vitro studies to investigate its enzymatic activity, structural features, and interactions. Produced using expression systems like *E. coli* or mammalian cells, the recombinant form retains functional domains, including two kinase domains (N-terminal and C-terminal) and a linker region critical for regulation. Its production enables detailed biochemical assays, inhibitor screening, and mechanistic studies in diseases like cancer, inflammation, and neurological disorders, where dysregulated RPS6KA5 signaling has been implicated.
Research on recombinant RPS6KA5 aids in understanding its role in stress responses, tumor progression, and immune regulation. It also serves as a tool for developing targeted therapies, as aberrant kinase activity is linked to pathologies such as breast cancer and rheumatoid arthritis. The recombinant protein’s purity and activity are validated to ensure reliability in experimental models, supporting both basic research and translational applications.
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