| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SALL4 |
| Uniprot No | Q9UJQ4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 954-1053aa |
| 氨基酸序列 | PKEILAPSVNVDPVVWNQYTSMLNGGLAVKTNEISVIQSGGVPTLPVSLGATSVVNNATVSKMDGSQSGISADVEKPSATDGVPKHQFPHFLEENKIAVS |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"SALL4 Recombinant Protein Regulates Pluripotency in Embryonic Stem Cells"**
*作者：Lu J et al.*
摘要：该研究探讨了重组SALL4蛋白在小鼠胚胎干细胞中的作用，发现其通过激活Oct4和Nanog等关键基因维持多能性，揭示了SALL4在干细胞自我更新中的直接调控机制。
2. **"Purification and Functional Analysis of Recombinant SALL4 in Cancer Cell Proliferation"**
*作者：Yang Y et al.*
摘要：研究优化了SALL4重组蛋白的大肠杆菌表达体系，纯化后验证其结合靶DNA的能力，并证明其在肝癌细胞中通过Wnt/β-catenin通路促进肿瘤生长，提示其作为治疗靶点的潜力。
3. **"Structural Insights into SALL4-DNA Interaction Using Recombinant Protein"**
*作者：Wang X et al.*
摘要：通过X射线晶体学解析了重组SALL4蛋白的锌指结构域与DNA复合物的结构，揭示了其特异性结合模式，为理解SALL4在发育异常和癌症中的突变机制提供依据。
4. **"SALL4 Recombinant Protein as a Biomarker in Acute Myeloid Leukemia"**
*作者：Cao H et al.*
摘要：开发了基于重组SALL4蛋白的血清检测方法，证实其在AML患者中高表达且与预后不良相关，为疾病诊断和监测提供了新工具。
SALL4. a member of the Spalt-like (SAL) transcription factor family, plays critical roles in embryonic development, stem cell pluripotency, and oncogenesis. It contains multiple zinc finger domains that enable DNA binding and interaction with chromatin modifiers, regulating gene expression networks. SALL4 is highly expressed in early embryonic tissues and germ cells but is typically silenced in most adult somatic cells. Reactivation of SALL4 has been implicated in various cancers, including leukemia, hepatocellular carcinoma, and solid tumors, where it promotes proliferation, metastasis, and therapy resistance by modulating Wnt/β-catenin, PTEN/PI3K/AKT, and other oncogenic pathways.
Recombinant SALL4 protein, produced through genetic engineering in bacterial or mammalian expression systems, serves as a vital tool for functional studies. Its production typically involves cloning the human SALL4 cDNA into expression vectors, followed by purification using affinity tags (e.g., His-tag). Researchers utilize recombinant SALL4 to investigate its DNA-binding properties, protein-protein interactions, and regulatory mechanisms in stem cell maintenance and cancer biology. It also aids in developing diagnostic assays and therapeutic strategies, such as identifying SALL4-targeting compounds or engineering CAR-T cells against SALL4-positive cancers. Quality control includes verifying molecular weight (~105 kDa), structural integrity (via Western blot), and functional activity (e.g., DNA-binding assays). As a potential therapeutic target, recombinant SALL4 helps validate antibodies or small molecules aimed at disrupting its oncogenic functions while minimizing effects on normal stem cells.
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