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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FGF16 |
| Uniprot No | O43320 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-207aa |
| 氨基酸序列 | MAEVGGVFAS LDWDLHGFSS SLGNVPLADS PGFLNERLGQ IEGKLQRGSP TDFAHLKGIL RRRQLYCRTG FHLEIFPNGT VHGTRHDHSR FGILEFISLA VGLISIRGVD SGLYLGMNER GELYGSKKLT RECVFREQFE ENWYNTYAST LYKHSDSERQ YYVALNKDGS PREGYRTKRH QKFTHFLPRP VDPSKLPSMS RDLFHYR |
| 预测分子量 | 23,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FGF16重组蛋白的模拟参考文献示例(仅供参考，非真实文献)：
1. **文献名称**：*Recombinant FGF16 promotes cardiac progenitor cell proliferation via MAPK signaling*
**作者**：Yamamoto K, et al.
**摘要**：研究通过大肠杆菌系统成功表达并纯化重组人FGF16蛋白，证实其可激活MAPK信号通路，促进心脏祖细胞体外增殖，提示其在心脏修复中的潜在应用。
2. **文献名称**：*Structural and functional characterization of FGF16 in brown adipose tissue thermogenesis*
**作者**：Zhang L, et al.
**摘要**：利用哺乳动物细胞表达重组FGF16.解析其蛋白结构，发现其通过结合FGFR1受体增强棕色脂肪细胞产热活性，为代谢性疾病治疗提供新靶点。
3. **文献名称**：*FGF16 recombinant protein rescues developmental defects in a zebrafish model*
**作者**：Smith J, et al.
**摘要**：研究利用重组FGF16蛋白处理FGF16基因敲除斑马鱼胚胎，显著改善心脏发育异常，证实其在胚胎组织分化中的关键作用。
4. **文献名称**：*High-yield production of bioactive FGF16 in Pichia pastoris*
**作者**：Chen H, et al.
**摘要**：开发毕赤酵母表达系统高效生产重组FGF16.优化纯化工艺，并验证其刺激成纤维细胞迁移的能力，为规模化制备奠定基础。
(注：以上为模拟内容，实际文献需通过学术数据库检索。)
Fibroblast Growth Factor 16 (FGF16) is a member of the FGF family, a group of signaling proteins involved in diverse biological processes, including embryonic development, tissue repair, metabolism, and carcinogenesis. FGF16 belongs to the FGF9 subfamily (FGF9/16/20) and shares structural homology with other paracrine-acting FGFs, featuring a conserved β-trefoil core domain and heparin-binding regions critical for receptor interaction. It is primarily secreted as a glycosylated protein, though recombinant forms are often produced in *E. coli* or mammalian expression systems for research purposes.
FGF16 plays key roles in organogenesis, particularly in cardiac development, brain patterning, and hair follicle morphogenesis. Studies highlight its involvement in regulating cardiomyocyte proliferation, neural cell differentiation, and Wnt/β-catenin signaling pathways. Unlike endocrine FGFs (e.g., FGF21/FGF23), FGF16 acts locally, requiring cell surface heparan sulfate proteoglycans to activate FGF receptors (FGFR1-4), often in a tissue-specific manner.
Recombinant FGF16 is generated via cloning and overexpression of the human or murine *Fgf16* gene, followed by purification techniques like affinity chromatography. Its applications span *in vitro* studies (e.g., stem cell differentiation, tissue engineering) and *in vivo* disease models, particularly for heart regeneration and developmental disorders. Dysregulation of FGF16 has been linked to cancers (e.g., breast, thyroid) and congenital heart defects, underscoring its therapeutic and diagnostic potential. Current research focuses on optimizing its stability, delivery mechanisms, and receptor specificity to advance regenerative medicine and targeted therapies.
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