| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CK-BB |
| Uniprot No | Q14204 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | aa |
| 氨基酸序列 | ; |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CK-BB重组蛋白的示例参考文献(注：部分文献信息为示例性质，建议通过学术数据库核实具体内容)：
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1. **"Expression and purification of recombinant human brain-type creatine kinase (CK-BB) in Escherichia coli"**
*Yamashita T. et al., 1995*
摘要：本研究成功在大肠杆菌中克隆并表达了人源CK-BB重组蛋白，通过亲和层析纯化获得高纯度蛋白，并验证其酶活性与天然CK-BB相似，为临床检测提供了可靠工具。
2. **"Development of a CK-BB-specific immunoassay using recombinant protein for neurological disorder diagnosis"**
*Smith J.L. et al., 2001*
摘要：利用重组CK-BB蛋白开发了一种高灵敏度免疫分析法，证实其在脑损伤和神经退行性疾病患者血清中的诊断潜力，优于传统检测方法。
3. **"Characterization of recombinant CK-BB as a biomarker for small cell lung cancer"**
*Johnson R.K. & Patel M., 2008*
摘要：通过哺乳动物细胞表达系统制备重组CK-BB，发现其在肺癌患者血清中异常升高，提示其作为肿瘤标志物的潜在价值。
4. **"Structural and functional analysis of CK-BB isoforms via recombinant protein technology"**
*Lee S. et al., 2012*
摘要：利用重组CK-BB蛋白解析其晶体结构，揭示不同异构体的酶动力学差异，为靶向药物设计提供结构基础。
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**建议**：以上为模拟示例，实际文献可通过PubMed、Web of Science等平台检索关键词“CK-BB recombinant protein”“creatine kinase BB expression”获取。近年研究可能侧重其在癌症/神经疾病中的机制或检测技术优化。
**Background of CK-BB Recombinant Protein**
Creatine kinase BB (CK-BB), also known as **CK-Brain type**, is an isoform of the creatine kinase (CK) enzyme family, which plays a critical role in cellular energy homeostasis by catalyzing the reversible transfer of phosphate groups between creatine and adenosine triphosphate (ATP). CK-BB is predominantly expressed in brain tissue, smooth muscle, and other non-muscle tissues, distinguishing it from the muscle-specific CK-MM and the hybrid CK-MB isoforms.
The recombinant CK-BB protein is produced through genetic engineering techniques, where the human *CKB* gene is cloned and expressed in heterologous systems like *E. coli* or mammalian cell cultures. This ensures high purity and consistency, making it a valuable tool for research and diagnostics. Recombinant CK-BB retains the enzymatic activity and structural features of the native protein, enabling its use in functional studies.
Clinically, CK-BB has been explored as a biomarker for brain injury, neurodegenerative diseases, and certain cancers, including prostate and lung cancers, where elevated levels correlate with disease progression. However, its low serum concentration in healthy individuals and rapid clearance limit its diagnostic utility without highly sensitive detection methods.
In research, recombinant CK-BB aids in elucidating mechanisms of cellular energy metabolism, neuroprotection, and tumor biology. It is also used to develop antibodies for immunoassays or as a reference standard in biomarker quantification.
Despite its niche applications, challenges remain in optimizing stability and activity in vitro. Ongoing studies focus on enhancing its therapeutic potential, such as in mitigating ischemic injury by buffering ATP levels. Overall, CK-BB recombinant protein serves as a vital reagent bridging biochemical research and clinical innovation.
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