| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCK18 |
| Uniprot No | P06307 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 46-103aa |
| 氨基酸序列 | VSQRTDGESRAHLGALLARYIQQARKAPSGRMSIVKNLQNLDPSHRISDRDYMGWMDF |
| 预测分子量 | 12,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCK18重组蛋白的假设参考文献示例(注：实际文献可能有限，建议结合具体需求进一步检索)：
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1. **标题**: *Expression and Functional Characterization of Recombinant CCK18 in Mammalian Cells*
**作者**: Zhang L, et al.
**摘要**: 本研究利用HEK293细胞系统成功表达了重组CCK18蛋白，并通过亲和层析纯化。功能实验表明，CCK18能激活胆囊收缩素受体CCK1R，并促进胰岛素分泌，提示其在代谢调控中的潜在作用。
2. **标题**: *Optimization of CCK18 Recombinant Production in E. coli for Neurobiological Studies*
**作者**: Müller T, et al.
**摘要**: 通过大肠杆菌表达系统优化CCK18的溶解性和产量，获得高纯度重组蛋白。动物模型实验显示，CCK18可通过血脑屏障并影响中枢食欲调控，为神经肽研究提供新工具。
3. **标题**: *CCK18 Recombinant Protein Attenuates Colitis in a Murine Model via Anti-inflammatory Pathways*
**作者**: Kim S, et al.
**摘要**: 重组CCK18在炎症性肠病模型中显著降低肠道炎症因子(如TNF-α、IL-6)，机制涉及TLR4/NF-κB信号通路抑制，表明其治疗肠道疾病的潜力。
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**注意**：以上文献为示例，实际研究中CCK18相关文献较少，可能需确认术语准确性(如是否为CCK8、CCK33或CCL18)。建议使用PubMed、Google Scholar等平台，以“CCK18 recombinant protein”、“Cholecystokinin-18 expression”等关键词检索最新研究。
**Background of CCK18 Recombinant Protein**
Cholecystokinin-18 (CCK18) is a bioactive peptide derived from the larger precursor protein preprocholecystokinin, which is primarily expressed in the gastrointestinal tract and central nervous system. As a member of the cholecystokinin (CCK) family, CCK18 shares structural and functional homology with other CCK isoforms, such as CCK-8 and CCK-33. but is characterized by its 18-amino-acid sequence. A distinguishing feature of CCK18 is the sulfated tyrosine residue at position 7. critical for binding and activating CCK receptors (CCK1R and CCK2R), which mediate its physiological effects.
CCK18 plays dual roles as a gastrointestinal hormone and a neuropeptide. In the gut, it regulates digestion by stimulating pancreatic enzyme secretion, gallbladder contraction, and gut motility. In the brain, it modulates satiety, anxiety, and pain perception. Dysregulation of CCK signaling is implicated in disorders like irritable bowel syndrome, obesity, and neurodegenerative diseases.
The recombinant CCK18 protein is engineered using biotechnological platforms (e.g., E. coli or mammalian cell systems) to ensure high purity and bioactivity. Recombinant production overcomes challenges associated with natural extraction, such as low yield and structural heterogeneity. It retains post-translational modifications essential for receptor interaction, making it a valuable tool for studying CCK receptor pharmacology, signal transduction, and therapeutic potential.
In research, CCK18 recombinant protein is utilized in vitro and in vivo to investigate its roles in appetite regulation, gut-brain axis communication, and disease mechanisms. Its application extends to drug discovery, particularly in developing receptor agonists or antagonists for metabolic or neurological disorders. By providing a consistent and scalable source, recombinant CCK18 facilitates both basic science and translational studies aimed at harnessing CCK pathways for clinical interventions.
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