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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | bEP |
| Uniprot No | Q8WWY8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-451aa |
| 氨基酸序列 | EE TCPSFTRLSF HSAVVGTGLN VRLMLYTRKN LTCAQTINSS AFGNLNVTKK TTFIVHGFRP TGSPPVWMDD LVKGLLSVED MNVVVVDWNR GATTLIYTHA SSKTRKVAMV LKEFIDQMLA EGASLDDIYM IGVSLGAHIS GFVGEMYDGW LGRITGLDPA GPLFNGKPHQ DRLDPSDAQF VDVIHSDTDA LGYKEPLGNI DFYPNGGLDQ PGCPKTILGG FQYFKCDHQR SVYLYLSSLR ESCTITAYPC DSYQDYRNGK CVSCGTSQKE SCPLLGYYAD NWKDHLRGKD PPMTKAFFDT AEESPFCMYH YFVDIITWNK NVRRGDITIK LRDKAGNTTE SKINHEPTTF QKYHQVSLLA RFNQDLDKVA AISLMFSTGS LIGPRYKLRI LRMKLRSLAH PERPQLCRYD LVLMENVETV FQPILCPELQ L |
| 预测分子量 | 50,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于bEP(牛胚胎蛋白)重组蛋白的模拟参考文献示例(注：文献为虚构示例，实际研究请通过学术数据库检索)：
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1. **文献名称**: *Expression and purification of recombinant bovine embryonic protein (bEP) in E. coli for biomedical applications*
**作者**: Zhang, L., et al.
**摘要**: 本研究通过大肠杆菌表达系统成功表达bEP重组蛋白，优化纯化工艺后获得高纯度产物。实验表明重组bEP在体外细胞培养中促进胚胎干细胞增殖，提示其在再生医学中的潜在应用。
2. **文献名称**: *Functional characterization of bEP in bovine reproductive physiology: A recombinant protein approach*
**作者**: Martinez, R., & Thompson, J.
**摘要**: 利用重组bEP蛋白研究其在牛胚胎早期发育中的作用，发现其通过激活MAPK信号通路调节胚胎着床过程，为改善家畜繁殖效率提供理论依据。
3. **文献名称**: *Development of a bEP-based ELISA for early pregnancy diagnosis in cattle*
**作者**: Singh, A., et al.
**摘要**: 基于重组bEP蛋白开发牛早期妊娠诊断试剂盒，临床样本验证显示其敏感性和特异性均优于传统方法，可显著降低畜牧业的繁殖管理成本。
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**建议**：
实际研究中，可通过PubMed或Google Scholar检索关键词如“recombinant bovine embryonic protein”、“bEP protein application”等获取真实文献。注意结合具体研究领域(如生殖生物学、蛋白质工程)筛选文献。
The bEP (bacterial-expressed recombinant protein) represents a class of biotechnologically engineered proteins produced using bacterial expression systems, most commonly *Escherichia coli*. This approach leverages the efficiency and cost-effectiveness of bacterial hosts to synthesize proteins of interest, which are often challenging to isolate from their native sources. The concept emerged in the 1980s with advances in molecular cloning, enabling the insertion of target gene sequences into plasmid vectors under inducible promoters. Upon transformation into bacterial hosts, these vectors direct protein production during fermentation.
bEP technology gained prominence due to its scalability and rapid turnaround. Unlike eukaryotic systems, bacterial platforms lack post-translational modification capabilities, making them ideal for producing simple, non-glycosylated proteins. Applications span therapeutic agents (e.g., insulin, growth factors), diagnostic reagents, and industrial enzymes. For instance, human insulin produced via *E. coli* revolutionized diabetes treatment, replacing animal-derived insulin and reducing immunogenicity risks.
However, challenges persist. Bacterial systems may generate inclusion bodies—aggregates of misfolded proteins—requiring complex refolding steps. Additionally, endotoxin removal is critical for therapeutic use. Recent innovations, such as codon optimization, fusion tags, and engineered strains (e.g., BL21(DE3)), have enhanced solubility and yield.
The bEP framework continues to evolve with synthetic biology tools, enabling precise control over expression conditions and protein engineering. Its role in pandemic responses (e.g., recombinant spike proteins for COVID-19 research) underscores its adaptability. While mammalian and yeast systems dominate complex protein production, bEP remains a cornerstone for high-volume, cost-sensitive applications, reflecting its enduring impact on biomanufacturing and biomedical research.
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