| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | EM2 |
| Uniprot No | P0DX32 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-14aa |
| 氨基酸序列 | LKLLGIVKKVLGAI |
| 预测分子量 | 1,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EM2重组蛋白的3篇示例参考文献(注：EM2重组蛋白的文献可能较少，以下内容为模拟示例，建议通过学术数据库获取真实文献)：
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1. **文献名称**：*Expression and Functional Analysis of EM2 Recombinant Protein in Tumor Metastasis*
**作者**：Zhang L, et al. (2020)
**摘要**：研究通过大肠杆菌系统成功表达EM2重组蛋白，并发现其通过激活TGF-β信号通路促进乳腺癌细胞的迁移和侵袭，为癌症治疗提供潜在靶点。
2. **文献名称**：*Purification and Structural Characterization of EM2 Recombinant Protein from Yeast Expression System*
**作者**：Wang Y, et al. (2018)
**摘要**：利用毕赤酵母表达系统高效生产EM2重组蛋白，通过色谱技术纯化并解析其三维结构，证实其与天然蛋白具有相似的生物活性。
3. **文献名称**：*EM2 Recombinant Protein Modulates Macrophage Polarization in Inflammatory Diseases*
**作者**：Chen H, et al. (2021)
**摘要**：实验表明，EM2重组蛋白可诱导巨噬细胞向抗炎表型(M2型)转化，减轻小鼠模型中的炎症反应，提示其在免疫治疗中的应用潜力。
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**备注**：以上内容为示例性概括，实际文献需通过PubMed、Web of Science或CNKI等平台检索。若需具体领域(如神经科学、感染免疫等)的研究，建议补充关键词进一步筛选。
EM2 (Epimorphin), also known as syntaxin-2 or EPIM, is a secreted protein first identified in the 1990s for its role in epithelial morphogenesis. Structurally, it belongs to the syntaxin family of membrane-trafficking proteins but exhibits unique extracellular functions. With a molecular weight of ~34 kDa, EM2 contains a conserved SNARE homology domain, though its secretion mechanism remains partially understood. It is widely expressed in epithelial and stromal cells across tissues like lung, liver, and mammary glands.
Functionally, EM2 acts as a versatile regulator of tissue architecture. It orchestrates lumen formation, branching morphogenesis, and cell polarity through interactions with extracellular matrix components and cell surface receptors. Studies highlight its dual role in cellular processes: promoting epithelial differentiation under physiological conditions while paradoxically enhancing tumor aggressiveness in cancers like colorectal and hepatocellular carcinoma. Its involvement in Wnt/β-catenin and TGF-β signaling pathways underscores its importance in development and disease.
Recombinant EM2 production typically employs E. coli or mammalian expression systems, enabling research applications in 3D organoid culture, wound healing models, and cancer biology. Recent investigations explore its therapeutic potential in regenerative medicine and as a biomarker for epithelial-derived malignancies. Despite progress, debates persist regarding its intracellular vs. extracellular mechanisms and context-dependent roles in tumor microenvironments. Ongoing research aims to resolve these ambiguities while developing EM2-targeted strategies for tissue engineering and precision oncology.
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