Recombinant Human DHA protein

中文名： 二十二碳六烯酸(DHA)牛血清白蛋白偶联物
别名： DHA；ALDH5；ALDHX；Aldehyde dehydrogenase X, mitochondrial

货号: PA2000-1019

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	DHA
Uniprot No	P30837
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	18-517aa
氨基酸序列	RYS SAAALPSPIL NPDIPYNQLF INNEWQDAVS KKTFPTVNPT TGEVIGHVAE GDRADVDRAV KAAREAFRLG SPWRRMDASE RGRLLNLLAD LVERDRVYLA SLETLDNGKP FQESYALDLD EVIKVYRYFA GWADKWHGKT IPMDGQHFCF TRHEPVGVCG QIIPWNFPLV MQGWKLAPAL ATGNTVVMKV AEQTPLSALY LASLIKEAGF PPGVVNIITG YGPTAGAAIA QHVDVDKVAF TGSTEVGHLI QKAAGDSNLK RVTLELGGKS PSIVLADADM EHAVEQCHEA LFFNMGQCCC AGSRTFVEES IYNEFLERTV EKAKQRKVGN PFELDTQQGP QVDKEQFERV LGYIQLGQKE GAKLLCGGER FGERGFFIKP TVFGGVQDDM RIAKEEIFGP VQPLFKFKKI EEVVERANNT RYGLAAAVFT RDLDKAMYFT QALQAGTVWV NTYNIVTCHT PFGGFKESGN GRELGEDGLK AYTEVKTVTI KVPQKNS
预测分子量	57,2 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于DHA重组蛋白研究的模拟参考文献示例(文献信息为假设，供参考)：

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1. **文献名称**：*Heterologous Expression of Schizochytrium PKS Pathway in E. coli for DHA Production*

**作者**：Hauvermale A., et al.

**摘要**：本研究通过重组技术将裂殖壶菌(*Schizochytrium*)中合成DHA的多酮合酶(PKS)基因簇导入大肠杆菌，成功实现DHA的异源合成，为微生物发酵生产DHA提供了新策略。

2. **文献名称**：*Structural and Functional Analysis of a Marine Bacterial DHA Synthase*

**作者**：Nichols D.S., et al.

**摘要**：解析了海洋细菌中DHA合成关键酶的三维结构，并通过重组蛋白表达验证了其催化功能，揭示了DHA生物合成的分子机制。

3. **文献名称**：*Engineering Saccharomyces cerevisiae for DHA Production via Recombinant Polyketide Synthases*

**作者**：Pereira H., et al.

**摘要**：利用酵母重组系统表达优化的PKS酶，显著提高了DHA产量，并评估了重组蛋白的稳定性及代谢通路的效率。

4. **文献名称**：*Functional Characterization of Recombinant Elovl5 Enzyme in DHA Biosynthesis*

**作者**：Metz J.G., et al.

**摘要**：克隆并表达了延长酶Elovl5的重组蛋白，证实其在DHA前体脂肪酸延伸中的关键作用，为代谢工程改造提供了理论基础。

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**注意**：以上文献为示例，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。关键词建议：**DHA biosynthesis, recombinant polyketide synthase, heterologous expression, Elovl5 enzyme**。

背景信息

DHA (docosahexaenoic acid) recombinant protein is a product of advanced biotechnology aimed at addressing the growing demand for sustainable sources of omega-3 fatty acids. DHA, a long-chain polyunsaturated fatty acid (LC-PUFA), is critical for brain development, cardiovascular health, and anti-inflammatory functions. Traditionally, it is sourced from marine organisms like fish oil or algae, but these methods face challenges such as overfishing, environmental contaminants, and inconsistent supply. Recombinant protein technology offers an alternative by leveraging genetically engineered microorganisms or cell lines to produce DHA-associated proteins or enzymes involved in its biosynthesis.

The development of DHA recombinant proteins often focuses on key enzymes in the DHA synthesis pathway, such as desaturases and elongases, which convert precursor fatty acids into DHA. For instance, genes encoding these enzymes from marine microbes (e.g., *Schizochytrium* or *Thraustochytrids*) are cloned into heterologous hosts like *Escherichia coli*, yeast, or plant systems. Metabolic engineering optimizes these hosts to enhance enzyme activity and DHA yield. This approach not only improves production efficiency but also reduces reliance on marine resources.

Applications span nutraceuticals, infant formula, pharmaceuticals, and aquaculture feed. Recombinant DHA proteins enable scalable, contaminant-free production with customizable fatty acid profiles. However, challenges remain, including high fermentation costs, low enzyme stability, and regulatory hurdles for genetically modified organisms (GMOs). Ongoing research integrates synthetic biology and fermentation optimization to overcome these barriers. As synthetic biology advances, DHA recombinant proteins are poised to play a pivotal role in sustainable omega-3 supply chains, aligning with global goals for ecological conservation and food security.