| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCS |
| Uniprot No | Q96RD7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-426aa |
| 氨基酸序列 | MAIAQLATEYVFSDFLLKEPTEPKFKGLRLELAVDKMVTCIAVGLPLLLISLAFAQEISIGTQISCFSPSSFSWRQAAFVDSYCWAAVQQKNSLQSESGNLPLWLHKFFPYILLLFAILLYLPPLFWRFAAAPHICSDLKFIMEELDKVYNRAIKAAKSARDLDMRDGACSVPGVTENLGQSLWEVSESHFKYPIVEQYLKTKKNSNNLIIKYISCRLLTLIIILLACIYLGYYFSLSSLSDEFVCSIKSGILRNDSTVPDQFQCKLIAVGIFQLLSVINLVVYVLLAPVVVYTLFVPFRQKTDVLKVYEILPTFDVLHFKSEGYNDLSLYNLFLEENISEVKSYKCLKVLENIKSSGQGIDPMLLLTNLGMIKMDVVDGKTPMSAEMREEQGNQTAELQGMNIDSETKANNGEKNARQRLLDSSC |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TCS重组蛋白的3篇代表性文献的简要总结(注：文献为虚构示例，仅供格式参考)：
1. **文献名称**: "High-yield expression and functional characterization of recombinant TCS protein in Pichia pastoris"
**作者**: Chen, X., Wang, Y., & Liu, H. (2021)
**摘要**: 本研究利用毕赤酵母系统高效表达TCS(双组分信号转导系统)重组蛋白，优化了发酵条件并实现高纯度蛋白制备。通过体外磷酸化实验验证了其激酶活性，为病原微生物中TCS信号通路研究提供工具。
2. **文献名称**: "Crystal structure analysis of TCS recombinant protein reveals novel dimerization interface"
**作者**: Singh, R., Kumar, A., & Patel, S. (2019)
**摘要**: 通过X射线晶体学解析了TCS重组蛋白的3D结构，发现其独特的二聚化界面及ATP结合域构象变化，揭示了该蛋白在细菌耐药性调控中的潜在分子机制。
3. **文献名称**: "TCS recombinant protein as a serodiagnostic antigen for tuberculosis detection"
**作者**: Gómez, L., et al. (2020)
**摘要**: 评估重组TCS蛋白在结核病血清学诊断中的应用，证实其与患者抗体高特异性结合，敏感度达92%，显著优于传统PPD抗原，具有临床转化潜力。
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**注**：以上文献为模拟示例，实际研究中建议通过PubMed、Web of Science等数据库检索真实文献。若需具体领域文献，可进一步限定研究背景(如TCS蛋白的生物学功能或应用方向)。
TCS recombinant proteins are engineered proteins produced through recombinant DNA technology, a cornerstone of modern biotechnology. These proteins are derived from the genetic modification of host organisms, such as bacteria (e.g., *E. coli*), yeast, or mammalian cells, to express specific target proteins encoded by inserted genes. The term "TCS" may refer to a proprietary or context-specific protein, but broadly, recombinant proteins like those under the TCS designation are designed for research, therapeutic, or industrial applications. Their production involves isolating the gene of interest, cloning it into an expression vector, and introducing it into a host system for large-scale protein synthesis.
The development of recombinant protein technology emerged in the 1970s, revolutionizing biomedicine by enabling the production of proteins that are difficult to isolate from natural sources. TCS recombinant proteins, like others in this category, offer advantages such as high purity, consistency, and scalability. They are critical tools in drug discovery, vaccine development (e.g., insulin, monoclonal antibodies), and structural biology studies. For instance, TCS-branded proteins might serve as antigens, enzymes, or signaling molecules in diagnostic assays or as therapeutic agents targeting diseases like cancer or autoimmune disorders.
Quality control is paramount in their production. Techniques like chromatography and mass spectrometry ensure proper folding, post-translational modifications, and functionality. Challenges include optimizing expression conditions to enhance yield and minimizing host-induced contaminants. As biotechnology advances, TCS recombinant proteins exemplify the intersection of genetic engineering and industrial application, driving innovations in personalized medicine and biopharmaceuticals while adhering to stringent regulatory standards for safety and efficacy. Their versatility continues to expand their role in both basic research and clinical solutions.
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