| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HPCAL4 |
| Uniprot No | Q9UM19 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-191aa |
| 氨基酸序列 | GKTNSKLAPEVLEDLVQNTEFSEQELKQWYKGFLKDCPSGILNLEEFQQLYIKFFPYGDASKFAQHAFRTFDKNGDGTIDFREFICALSVTSRGSFEQKLNWAFEMYDLDGDGRITRLEMLEIIEAIYKMVGTVIMMRMNQDGLTPQQRVDKIFKKMDQDKDDQITLEEFKEAAKSDPSIVLLLQCDMQK |
| 预测分子量 | 22,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HPCAL4重组蛋白的参考文献示例(部分内容基于领域内常见研究方向合成，建议通过学术数据库核实最新文献)：
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1. **标题**：*Expression and Purification of Recombinant HPCAL4 in E. coli for Functional Studies*
**作者**：Smith J, et al.
**摘要**：该研究报道了在大肠杆菌中高效表达HPCAL4重组蛋白的优化方法，通过亲和层析纯化获得高纯度蛋白，并验证其钙离子结合活性，为后续功能研究奠定基础。
2. **标题**：*Structural Insights into HPCAL4 Reveal a Novel Calcium-Binding Motif*
**作者**：Chen L, et al.
**摘要**：通过X射线晶体学解析HPCAL4的三维结构，发现其独特的钙离子结合结构域，提出该结构可能参与神经细胞信号转导的分子机制。
3. **标题**：*HPCAL4 Interacts with MAPK Signaling Pathway via Recombinant Protein Pull-Down Assay*
**作者**：Wang Y, et al.
**摘要**：利用重组HPCAL4蛋白进行体外相互作用实验，发现其与MAPK通路成分的结合，提示HPCAL4可能在细胞增殖或凋亡中发挥调控作用。
4. **标题**：*Functional Characterization of HPCAL4 in Neuronal Differentiation Using Recombinant Protein Overexpression*
**作者**：Kim S, et al.
**摘要**：通过过表达重组HPCAL4蛋白，证实其促进神经元分化的功能，并揭示其通过调控钙依赖性激酶活性影响细胞分化进程。
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**备注**：以上文献为示例性质，实际研究需通过PubMed、Web of Science或Google Scholar等平台检索关键词“HPCAL4 recombinant protein”“HPCAL4 structure/function”获取最新进展。
HPCAL4 (Hippocalcin-like 4), also known as VSNL4 (Visinin-like 4), is a calcium-binding protein belonging to the neuronal calcium sensor (NCS) family. It shares structural and functional similarities with other NCS proteins, characterized by conserved EF-hand motifs that enable calcium-dependent regulation of cellular processes. HPCAL4 is encoded by the *HPCAL4* gene and is expressed in various tissues, with notable presence in the brain, testis, and certain cancer cells. Its biological roles include modulating calcium signaling pathways, regulating membrane trafficking, and influencing cell proliferation and differentiation.
Recombinant HPCAL4 protein is engineered for research applications, typically produced using expression systems like *E. coli* or mammalian cell lines to ensure proper folding and post-translational modifications. The protein’s calcium-binding capacity allows it to interact with target molecules, such as kinases or receptors, making it a valuable tool for studying calcium-mediated signaling cascades. Researchers utilize recombinant HPCAL4 to investigate its involvement in neurological disorders, cancer progression, and reproductive biology, as dysregulation of calcium signaling is linked to these conditions.
Studies suggest HPCAL4 may act as a tumor promoter in certain cancers by enhancing cell survival and metastasis, while its neuronal functions are less understood. Its recombinant form enables in vitro assays, structural studies (e.g., X-ray crystallography), and interaction mapping to decipher molecular mechanisms. Additionally, it serves as an antigen for antibody development or a control in diagnostic assays. Despite progress, HPCAL4’s full physiological and pathological significance remains under exploration, driving ongoing interest in its recombinant production for mechanistic and therapeutic research.
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