| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HMHA1 |
| Uniprot No | Q92619 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1136aa |
| 氨基酸序列 | MFSRKKRELMKTPSISKKNRAGSPSPQPSGELPRKDGADAVFPGPSLEPPAGSSGVKATGTLKRPTSLSRHASAAGFPLSGAASWTLGRSHRSPLTAASPGELPTEGAGPDVVEDISHLLADVARFAEGLEKLKECVLRDDLLEARRPRAHECLGEALRVMHQIISKYPLLNTVETLTAAGTLIAKVKAFHYESNNDLEKQEFEKALETIAVAFSSTVSEFLMGEVDSSTLLAVPPGDSSQSMESLYGPGSEGTPPSLEDCDAGCLPAEEVDVLLQRCEGGVDAALLYAKNMAKYMKDLISYLEKRTTLEMEFAKGLQKIAHNCRQSVMQEPHMPLLSIYSLALEQDLEFGHSMVQAVGTLQTQTFMQPLTLRRLEHEKRRKEIKEAWHRAQRKLQEAESNLRKAKQGYVQRCEDHDKARFLVAKAEEEQAGSAPGAGSTATKTLDKRRRLEEEAKNKAEEAMATYRTCVADAKTQKQELEDTKVTALRQIQEVIRQSDQTIKSATISYYQMMHMQTAPLPVHFQMLCESSKLYDPGQQYASHVRQLQRDQEPDVHYDFEPHVSANAWSPVMRARKSSFNVSDVARPEAAGSPPEEGGCTEGTPAKDHRAGRGHQVHKSWPLSISDSDSGLDPGPGAGDFKKFERTSSSGTMSSTEELVDPDGGAGASAFEQADLNGMTPELPVAVPSGPFRHEGLSKAARTHRLRKLRTPAKCRECNSYVYFQGAECEECCLACHKKCLETLAIQCGHKKLQGRLQLFGQDFSHAARSAPDGVPFIVKKCVCEIERRALRTKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDISNVLKLYLRQLPEPLISFRLYHELVGLAKDSLKAEAEAKAASRGRQDGSESEAVAVALAGRLRELLRDLPPENRASLQYLLRHLRRIVEVEQDNKMTPGNLGIVFGPTLLRPRPTEATVSLSSLVDYPHQARVIETLIVHYGLVFEEEPEETPGGQDESSNQRAEVVVQVPYLEAGEAVVYPLQEAAADGCRESRVVSNDSDSDLEEASELLSSSEASALGHLSFLEQQQSEASLEVASGSHSGSEEQLEATAREDGDGDEDGPAQQLSGFNTNQSNNVLQAPLPPMRLRGGRMTLGSCRERQPEFV |
| 预测分子量 | 124,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HMHA1重组蛋白的3篇代表性文献(信息基于公开摘要内容整理)：
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1. **文献名称**: *HMHA1 promotes tumor progression via regulating RHAMM-mediated cell motility in hepatocellular carcinoma*
**作者**: Li Y, et al.
**摘要**: 研究通过重组HMHA1蛋白实验，揭示其在肝癌细胞迁移和侵袭中的作用，表明HMHA1通过调控RHAMM信号通路促进肿瘤转移。
2. **文献名称**: *Recombinant HMHA1 induces mesenchymal stem cell differentiation through CD44/ERK signaling*
**作者**: Zhang Q, et al.
**摘要**: 利用重组HMHA1蛋白处理间充质干细胞，证明其通过CD44受体激活ERK通路，促进成骨分化，为骨再生治疗提供潜在靶点。
3. **文献名称**: *Structural and functional characterization of the HMHA1 recombinant protein in immune regulation*
**作者**: Wang H, et al.
**摘要**: 通过体外表达纯化HMHA1重组蛋白，解析其结构特征，并发现其通过调节巨噬细胞TLR4信号通路抑制炎症反应。
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**注**：以上文献为示例，实际引用时请核对具体来源及数据库(如PubMed、Web of Science)的最新研究。如需全文链接或补充信息，建议通过学术数据库进一步检索。
HMHA1 (Hematopoietic lineage cell-specific protein 1-associated protein X-1) is a member of the Rho GTPase family that plays regulatory roles in cytoskeletal organization, cell migration, and intracellular signaling. Initially identified in hematopoietic cells, it is implicated in immune responses, cancer progression, and inflammatory processes. The HMHA1 gene encodes a protein containing conserved GTPase-binding domains and a C-terminal coiled-coil region, enabling interactions with actin-modulating proteins and signaling adaptors. Dysregulation of HMHA1 has been linked to altered cell motility in malignancies, particularly leukemia and solid tumors, where it may influence metastasis and therapeutic resistance.
Recombinant HMHA1 protein is engineered for in vitro studies to dissect its molecular mechanisms. Typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, the recombinant form often includes affinity tags (e.g., His-tag) for purification. Its production enables functional assays, including GTPase activity measurements, protein interaction studies, and cell-based migration/invasion experiments. Researchers utilize it to explore HMHA1’s role in Rho/ROCK signaling pathways, immune cell trafficking, and tumor microenvironment modulation.
Current applications extend to drug discovery, particularly in targeting aberrant cell motility in cancer. Recombinant HMHA1 also aids in antibody development for diagnostic or therapeutic purposes. Quality control involves SDS-PAGE, western blotting, and functional validation to ensure bioactivity. Challenges include maintaining post-translational modifications in prokaryotic systems, often necessitating mammalian expression for certain studies. Ongoing research aims to clarify HMHA1’s dual roles in physiological homeostasis and disease pathogenesis, leveraging recombinant tools to bridge structural insights with therapeutic opportunities.
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