| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIPK3 |
| Uniprot No | Q9H422 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1215aa |
| 氨基酸序列 | MASQVLVYPPYVYQTQSSAFCSVKKLKVEPSSCVFQERNYPRTYVNGRNFGNSHPPTKGSAFQTKIPFNRPRGHNFSLQTSAVVLKNTAGATKVIAAQAQQAHVQAPQIGAWRNRLHFLEGPQRCGLKRKSEELDNHSSAMQIVDELSILPAMLQTNMGNPVTVVTATTGSKQNCTTGEGDYQLVQHEVLCSMKNTYEVLDFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTENADEYNFVRAYECFQHRNHTCLVFEMLEQNLYDFLKQNKFSPLPLKVIRPILQQVATALKKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKTVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGALEYDQIRYISQTQGLPGEQLLNVGTKSTRFFCKETDMSHSGWRLKTLEEHEAETGMKSKEARKYIFNSLDDVAHVNTVMDLEGSDLLAEKADRREFVSLLKKMLLIDADLRITPAETLNHPFVNMKHLLDFPHSNHVKSCFHIMDICKSHLNSCDTNNHNKTSLLRPVASSSTATLTANFTKIGTLRSQALTTSAHSVVHHGIPLQAGTAQFGCGDAFQQTLIICPPAIQGIPATHGKPTSYSIRVDNTVPLVTQAPAVQPLQIRPGVLSQTWSGRTQQMLVPAWQQVTPLAPATTTLTSESVAGSHRLGDWGKMISCSNHYNSVMPQPLLTNQITLSAPQPVSVGIAHVVWPQPATTKKNKQCQNRGILVKLMEWEPGREEINAFSWSNSLQNTNIPHSAFISPKIINGKDVEEVSCIETQDNQNSEGEARNCCETSIRQDSDSSVSDKQRQTIIIADSPSPAVSVITISSDTDEEETSQRHSLRECKGSLDCEACQSTLNIDRMCSLSSPDSTLSTSSSGQSSPSPCKRPNSMSDEEQESSCDTVDGSPTSDSSGHDSPFAESTFVEDTHENTELVSSADTETKPAVCSVVVPPVELENGLNADEHMANTDSICQPLIKGRSAPGRLNQPSAVGTRQQKLTSAFQQQHLNFSQVQHFGSGHQEWNGNFGHRRQQAYIPTSVTSNPFTLSHGSPNHTAVHAHLAGNTHLGGQPTLLPYPSSATLSSAAPVAHLLASPCTSRPMLQHPTYNISHPSGIVHQVPVGLNPRLLPSPTIHQTQYKPIFPPHSYIAASPAYTGFPLSPTKLSQYPYM |
| 预测分子量 | 133,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIPK3重组蛋白的示例参考文献(均为虚构，仅供示例参考)：
1. **文献名称**: *"Recombinant HIPK3 kinase domain purification and its role in apoptosis signaling"*
**作者**: Smith A, et al.
**摘要**: 本研究通过大肠杆菌表达系统成功纯化HIPK3激酶结构域重组蛋白，并验证其在体外磷酸化p53的能力，揭示HIPK3通过调控p53介导细胞凋亡的分子机制。
2. **文献名称**: *"Structural and functional analysis of HIPK3 recombinant protein in TGF-β pathway regulation"*
**作者**: Tanaka K, et al.
**摘要**: 利用杆状病毒系统表达全长HIPK3重组蛋白，结合晶体学分析发现其通过磷酸化Smad4调控TGF-β信号转导，为靶向HIPK3的疾病治疗提供结构基础。
3. **文献名称**: *"Development of a high-throughput assay for HIPK3 kinase activity using recombinant protein"*
**作者**: Johnson R, et al.
**摘要**: 报道一种基于荧光共振能量转移(FRET)的HIPK3激酶活性检测方法，通过哺乳细胞表达的重组HIPK3筛选出小分子抑制剂，证明其在神经退行性疾病模型中的潜在应用。
4. **文献名称**: *"HIPK3 recombinant protein interacts with c-Jun in oxidative stress response"*
**作者**: Müller S, et al.
**摘要**: 通过GST pull-down实验发现重组HIPK3与c-Jun直接结合，并在氧化应激条件下增强c-Jun的转录活性，提示HIPK3在细胞应激反应中的新功能。
(注：以上文献为模拟内容，实际研究中请通过PubMed或专业数据库查询真实文献。)
HIPK3 (Homeodomain-Interacting Protein Kinase 3) is a serine/threonine kinase belonging to the HIPK family, which plays critical roles in cellular processes such as proliferation, apoptosis, and DNA damage response. It interacts with transcription factors, including homeodomain proteins, to modulate gene expression and signaling pathways. HIPK3 is structurally characterized by a conserved kinase domain, a homeobox-interacting region, and a C-terminal regulatory domain. Unlike its homologs HIPK1 and HIPK2. HIPK3 exhibits distinct substrate preferences and subcellular localization, suggesting functional divergence within the family.
Recombinant HIPK3 protein is produced using expression systems (e.g., bacterial, insect, or mammalian cells) to study its biochemical properties, enzymatic activity, and interactions. Researchers employ it to investigate HIPK3's role in diseases, particularly cancer and neurodegenerative disorders. For example, HIPK3 has been implicated in regulating Wnt/β-catenin and p53 pathways, influencing tumor progression and neuronal survival. Its recombinant form enables in vitro kinase assays, structural studies, and screening for kinase inhibitors. Challenges in producing active HIPK3 include maintaining post-translational modifications (e.g., autophosphorylation) critical for function, often addressed through eukaryotic expression systems. Current research focuses on clarifying HIPK3's dual roles as a context-dependent tumor suppressor or promoter, and its potential as a therapeutic target. The protein's utility extends to modeling disease mechanisms and developing targeted therapies against HIPK3-associated pathologies.
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