| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIPK4 |
| Uniprot No | Q8NE63 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-616aa |
| 氨基酸序列 | MSTIQSETDCYDIIEVLGKGTFGEVAKGWRRSTGEMVAIKILKNDAYRNR IIKNELKLLHCMRGLDPEEAHVIRFLEFFHDALKFYLVFELLEQNLFEFQ KENNFAPLPARHIRTVTLQVLTALARLKELAIIHADLKPENIMLVDQTRC PFRVKVIDFGSASIFSEVRYVKEPYIQSRFYRAPEILLGLPFCEKVDVWS LGCVMAELHLGWPLYPGNNEYDQVRYICETQGLPKPHLLHAACKAHHFFK RNPHPDAANPWQLKSSADYLAETKVRPLERRKYMLKSLDQIETVNGGSVA SRLTFPDREALAEHADLKSMVELIKRMLTWESHERISPSAALRHPFVS MQQLRSAHETTHYYQLSLRSYRLSLQVEGKPPTPVVAAEDGTPYYCLAEE KEAAGMGSVAGSSPFFREEKAPGMQRAIDQLDDLSLQEAGHGLWGETCTN AVSDMMVPLKAAITGHHVPDSGPEPILAFYSSRLAGRHKARKPPAGSKSD SNFSNLIRLSQVSPEDDRPCRGSSWEEGEHLGASAEPLAILQRDEDGPNI DNMTMEAERPDPELFDPSSCPGEWLSEPDCTLESVRGPRAQGLPPRRSHQ HGPPRGATSFLQHVTGHH |
| 预测分子量 | 85 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIPK4重组蛋白的3篇参考文献示例(注：文献为示例性质，实际引用需核实真实来源)：
1. **文献名称**：HIPK4 regulates spermatogenesis through its kinase activity by phosphorylating SPATA16
**作者**：Zhang Y, et al.
**摘要**：研究利用重组表达的HIPK4蛋白，证明其通过磷酸化精子形成相关蛋白SPATA16调控精子发生，体外实验验证了其激酶活性对精子细胞发育的影响。
2. **文献名称**：Recombinant HIPK4 expression and its role in DNA damage response
**作者**：Wang L, et al.
**摘要**：通过大肠杆菌系统成功表达并纯化HIPK4重组蛋白，发现其参与DNA损伤修复通路，体外实验显示HIPK4磷酸化下游靶点BRCA1.增强细胞对化疗药物的敏感性。
3. **文献名称**：Structural insights into HIPK4 kinase domain and its inhibition
**作者**：Chen X, et al.
**摘要**：解析了HIPK4重组蛋白激酶结构域的晶体结构，筛选出小分子抑制剂，并在细胞模型中验证其对HIPK4活性的抑制作用，为相关疾病治疗提供潜在靶点。
Homeodomain-interacting protein kinase 4 (HIPK4) is a serine/threonine kinase belonging to the HIPK family, which includes HIPK1-3. Primarily expressed in the testis and brain, HIPK4 plays critical roles in cellular processes such as transcriptional regulation, DNA damage response, and germ cell development. Unlike other HIPK members, HIPK4 exhibits distinct substrate preferences and signaling pathways. It phosphorylates proteins involved in chromatin remodeling (e.g., SPAG5) and cytoskeletal organization, particularly during spermatogenesis, where it regulates acrosome formation and sperm head shaping. Studies in HIPK4-knockout mice reveal male infertility due to defective sperm maturation, highlighting its essential role in reproduction.
Recombinant HIPK4 protein is engineered using expression systems (e.g., bacteria, insect cells) to produce purified, biologically active kinase for functional studies. This tool enables researchers to analyze HIPK4's enzymatic activity, substrate interactions, and regulatory mechanisms in vitro. Recent research also implicates HIPK4 in cancer progression, with altered expression observed in gliomas and prostate cancers, suggesting potential therapeutic targeting. Its involvement in stress-responsive pathways and apoptosis regulation further expands its biomedical relevance. However, HIPK4's full range of biological functions remains under investigation, partly due to its tissue-specific expression patterns and overlapping roles with other kinases. Recombinant HIPK4 continues to facilitate drug discovery and mechanistic studies bridging reproductive biology, neurobiology, and oncology.
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