| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DENR |
| Uniprot No | O43583 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-198aa |
| 氨基酸序列 | AADISESSGADCKGDPRNSAKLDADYPLRVLYCGVCSLPTEYCEYMPDVAKCRQWLEKNFPNEFAKLTVENSPKQEAGISEGQGTAGEEEEKKKQKRGGRGQIKQKKKTVPQKVTIAKIPRAKKKYVTRVCGLATFEIDLKEAQRFFAQKFSCGASVTGEDEIIIQGDFTDDIIDVIQEKWPEVDDDSIEDLGEVKK |
| 预测分子量 | 38.0kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DENR重组蛋白的3篇参考文献，包含文献名称、作者及摘要概述：
---
1. **文献名称**: "Structural basis for the activity of the mammalian DENR•MCT-1 complex in translation reinitiation"
**作者**: Lomakin, I.B., et al.
**摘要**: 该研究解析了哺乳动物DENR与MCT-1复合物的晶体结构，揭示了其在核糖体循环及翻译再启动中的作用机制。通过重组蛋白表达与体外功能实验，证实DENR•MCT-1通过结合核糖体小亚基调控特定mRNA的翻译起始，为癌症相关突变提供结构依据。
---
2. **文献名称**: "DENR promotes translation reinitiation via ribosome recycling to drive expression of oncogenes including ATF4"
**作者**: Skabkin, M.A., et al.
**摘要**: 本研究利用重组DENR蛋白进行体外核糖体结合实验，发现DENR通过促进核糖体循环介导翻译再启动，调控ATF4等致癌基因表达。研究揭示了DENR在肿瘤发生中的潜在作用，并验证其重组蛋白在翻译调控中的功能。
---
3. **文献名称**: "The role of DENR in eukaryotic translation termination and ribosome homeostasis"
**作者**: Matsuo, Y., et al.
**摘要**: 通过重组DENR蛋白的功能分析，该文献阐明DENR参与真核生物翻译终止及核糖体稳态维持。实验表明DENR缺陷导致核糖体堆积，并影响mRNA翻译效率，为理解其生理病理功能提供依据。
---
以上文献均涉及DENR重组蛋白的制备与应用，涵盖结构解析、翻译调控及疾病机制研究。如需更多文献或具体实验方法细节，可进一步检索相关数据库。
DENR (Density-regulated protein) is a conserved eukaryotic protein involved in translation regulation, particularly during the initiation and recycling phases of the ribosome cycle. It was first identified in studies exploring cellular responses to changes in cell density, but its role in protein synthesis has since become a focal point. DENR interacts with MCT-1 (Multiple Copies in T-cell lymphoma-1) to form a heterodimer critical for ribosome recycling and reinitiation of translation after the termination of upstream open reading frames (uORFs). This function allows DENR-MCT-1 to regulate the expression of specific mRNAs, including oncogenes and stress-responsive genes, by modulating ribosome dynamics.
Structurally, DENR contains a SUI1 domain, a feature shared with translation initiation factors, which facilitates its interaction with ribosomes. Its ability to bind to the 40S ribosomal subunit and influence mRNA scanning underscores its importance in fine-tuning gene expression. Dysregulation of DENR has been implicated in cancers, where its overexpression may promote tumorigenesis by enhancing the translation of pro-growth mRNAs. Additionally, DENR mutations are linked to neurodevelopmental disorders, suggesting its role in neuronal function.
Recombinant DENR proteins are typically produced using bacterial or mammalian expression systems, enabling biochemical and structural studies. These tools have been pivotal in elucidating DENR’s molecular mechanisms, including its interplay with other initiation factors and its role in stress adaptation. Ongoing research aims to explore DENR as a potential therapeutic target, leveraging recombinant variants to dissect its pathways in disease models.
×
