| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CEP55 |
| Uniprot No | Q53EZ4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-464aa |
| 氨基酸序列 | MSSRSTKDLI KSKWGSKPSN SKSETTLEKL KGEIAHLKTS VDEITSGKGK LTDKERHRLL EKIRVLEAEK EKNAYQLTEK DKEIQRLRDQ LKARYSTTTL LEQLEETTRE GERREQVLKA LSEEKDVLKQ QLSAATSRIA ELESKTNTLR LSQTVAPNCF NSSINNIHEM EIQLKDALEK NQQWLVYDQQ REVYVKGLLA KIFELEKKTE TAAHSLPQQT KKPESEGYLQ EEKQKCYNDL LASAKKDLEV ERQTITQLSF ELSEFRRKYE ETQKEVHNLN QLLYSQRRAD VQHLEDDRHK TEKIQKLREE NDIARGKLEE EKKRSEELLS QVQFLYTSLL KQQEEQTRVA LLEQQMQACT LDFENEKLDR QHVQHQLHVI LKELRKARNQ ITQLESLKQL HEFAITEPLV TFQGETENRE KVAASPKSPT AALNESLVEC PKCNIQYPAT EHRDLLVHVE YCSK |
| 预测分子量 | 54,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CEP55重组蛋白的3篇文献示例(内容基于公开研究整理，非真实文献)：
1. **文献名称**：*"Structural insights into CEP55 oligomerization and its role in cytokinesis"*
**作者**：Zhang Y. et al.
**摘要**：通过重组表达人源CEP55蛋白，结合X射线晶体学解析其C末端结构域的三维结构，揭示了CEP55通过寡聚化介导胞质分裂的分子机制，并发现其与微管结合的关键区域。
2. **文献名称**：*"Recombinant CEP55 interaction with ESCRT-III proteins facilitates abscission in human cells"*
**作者**：Guizetti J. et al.
**摘要**：利用重组CEP55蛋白进行体外结合实验，证明CEP55直接结合ESCRT-III复合物亚基CHMP4B，阐明了CEP55在细胞分裂末期膜切割过程中的桥梁作用。
3. **文献名称**：*"Overexpression of recombinant CEP55 promotes oncogenic phenotypes in vitro"*
**作者**：Lee H.C. et al.
**摘要**：通过哺乳动物系统表达重组CEP55.发现其过表达导致细胞周期异常和基因组不稳定，提示CEP55在癌症发生中可能作为促癌因子。
4. **文献名称**：*"Purification and functional analysis of CEP55 phospho-mimetic mutants"*
**作者**：Wang X. et al.
**摘要**：开发了重组CEP55磷酸化模拟突变体，通过体外激酶实验验证其磷酸化修饰对细胞分裂终止阶段的调控功能。
(注：以上文献为示例性质，实际文献需通过PubMed/Google Scholar检索确认。)
CEP55 (Centrosomal Protein 55) is a centrosome- and microtubule-associated protein critical for regulating the final stages of cell division (cytokinesis). It plays a key role in ensuring accurate abscission, the process by which daughter cells physically separate during mitosis. CEP55 interacts with components of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery to mediate membrane fission at the midbody, a transient structure formed during cytokinesis. Dysregulation of CCEP55 expression or function has been linked to genomic instability, polyploidy, and carcinogenesis, as errors in abscission can lead to chromosomal missegregation and oncogenic transformation. Overexpression of CEP55 is observed in various cancers, including breast, liver, and lung tumors, and is often associated with poor prognosis.
Recombinant CEP55 protein is engineered for in vitro studies to dissect its structural and functional roles. Typically produced in bacterial (e.g., E. coli) or mammalian expression systems, the recombinant protein retains functional domains, such as its coiled-coil regions and microtubule-binding motifs. It is widely used to investigate protein-protein interactions, particularly with ESCRT complexes (e.g., ALIX, TSG101) and cell cycle regulators. Researchers also employ it to screen small-molecule inhibitors targeting CEP55 activity, develop diagnostic antibodies, or model cytokinesis defects in cell-free systems. Purification often involves affinity tags (e.g., His-tag) for ease of isolation. Studies using recombinant CEP55 have advanced our understanding of abscission mechanics and its implications in cancer biology, offering potential therapeutic avenues for malignancies driven by cytokinesis failures.
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