| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CASC5 |
| Uniprot No | Q8NG31 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | 265,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CASC5重组蛋白的示例性参考文献(注：部分文献信息为模拟，仅供参考)：
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1. **文献名称**: "Expression and Functional Characterization of Recombinant CASC5 in Human Cell Lines"
**作者**: Wang et al.
**摘要**: 本研究通过哺乳动物表达系统成功制备了重组CASC5蛋白，并验证其参与有丝分裂中染色体定位的功能，揭示了其与MIS12复合体的相互作用对纺锤体组装检查点的调控作用。
2. **文献名称**: "Purification and Structural Analysis of CASC5 Recombinant Protein for Kinetochore Studies"
**作者**: Tanaka et al.
**摘要**: 报道了利用杆状病毒-昆虫细胞系统表达并纯化重组CASC5蛋白，通过冷冻电镜解析其结构，阐明其作为支架蛋白在动粒复合体组装中的关键作用。
3. **文献名称**: "CASC5 Knockdown Impairs Centromere Localization via Disruption of Bub1 Signaling: Insights from Recombinant Protein Rescue Assays"
**作者**: Gupta et al.
**摘要**: 通过外源性添加重组CASC5蛋白，验证其可部分恢复CASC5缺陷细胞的动粒Bub1激酶活性，证明其在维持染色体稳定性中的功能依赖性。
4. **文献名称**: "Development of a CASC5-Specific Monoclonal Antibody Using Recombinant Antigen"
**作者**: Li et al.
**摘要**: 利用大肠杆菌表达的重组CASC5蛋白作为免疫原，成功制备高特异性抗体，应用于癌症组织样本中CASC5异常表达的检测及临床相关性分析。
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*注：以上文献为示例，实际研究中建议通过PubMed或Web of Science等数据库检索真实发表的文献。*
CASC5 (Cancer Susceptibility Candidate 5), also known as KNSTRN or KNL1. is a critical protein involved in mitotic checkpoint regulation and chromosomal segregation during cell division. It functions as a core component of the kinetochore, a multi-protein structure essential for proper spindle microtubule attachment and the spindle assembly checkpoint (SAC). CASC5 interacts with other SAC proteins, such as BUB1 and MAD2. to prevent premature anaphase onset until all chromosomes achieve bipolar attachment, ensuring genomic stability. Dysregulation of CASC5 has been linked to chromosomal instability, a hallmark of cancer, and mutations in its encoding gene are associated with developmental disorders and tumorigenesis.
Recombinant CASC5 protein is engineered using heterologous expression systems (e.g., E. coli or mammalian cell lines) to produce purified, biologically active forms of the protein for research applications. Its recombinant form typically retains functional domains, including the conserved RWD and coiled-coil regions critical for kinetochore localization and protein-protein interactions. Researchers utilize recombinant CASC5 to study its role in mitosis, SAC signaling, and cancer mechanisms. It also serves as an antigen for antibody development or a tool for in vitro binding assays to map interaction networks within the kinetochore complex. Additionally, recombinant CASC5 is valuable in screening potential therapeutic compounds targeting mitotic errors in cancer cells. Studies have highlighted its potential as a biomarker for certain cancers, emphasizing its dual utility in basic research and translational oncology.
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