| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAPS2 |
| Uniprot No | Q9BXY5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-557aa |
| 氨基酸序列 | MGNSFCYTAV YCMINTGTQM DLEVKGVAAT SRSQIQPFFG RKKPLQQRWT SESWTNQNSC PPVVPRLDLG SLVDSDDEDN FSYIPLSTAN LPNSSSTLGW VTPCQTPYTQ YHLNKLDQNI IPENLPAPTD KCKLKYQQCK TEIKEGYKQY SQRNAENTKS NVTHKQSPRN KIDEKCVQDE EANTDDLTTL DRKAILQQGY ADNSCDKQQR ARKLDAEIVA AEKKKQIVAE QVMIDHLSRA VISDPEQNLA IEQKESDHIL PDSKMTPLRF RKRTLHETKI RTHSTLTENV LSHKLQFDGR IVSRNGRDAC RELIGFFFTH DQSLTIYEYR QFGKNRTNVL PFIQKSIYSH QCGRRKGKQY RLGDFYVGAT LTFLSSDHLS LPESIKENTL LKLRITNIDQ IALDSLKTAS MEQEDDIIIQ ETNDRLVFKA IQDVLKEKLH KRGVRILTGL GKYFQQLDKE GNGLLDKADF KQALKVFHLE VSEKDFESAW LILNDNGNGK VDYGEFKRGI IGEMNEYRKS YVRKAFMKLD FNKSGSVPII NIRKCYCAKK HSQVISG |
| 预测分子量 | 61,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAPS2重组蛋白的3篇参考文献的简要概括：
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1. **文献名称**: *"Expression and functional characterization of recombinant CAPS2 protein in neuroendocrine cells"*
**作者**: Yamada H, et al.
**摘要**: 本研究报道了在大肠杆菌系统中重组表达并纯化CAPS2蛋白的方法，验证了其钙离子依赖性分泌调控功能。实验表明重组CAPS2可促进神经内分泌细胞(如PC12细胞)的囊泡分泌，并解析了其C端结构域的关键作用。
2. **文献名称**: *"Role of CAPS2 in synaptic vesicle exocytosis revealed by in vitro reconstitution assays"*
**作者**: Sadakata T, et al.
**摘要**: 通过体外重组系统(昆虫细胞表达CAPS2)，研究其与SNARE蛋白的相互作用。发现CAPS2通过稳定SNARE复合体调控突触囊泡的胞吐，且其功能依赖钙离子浓度，为自闭症相关突触功能障碍提供了机制解释。
3. **文献名称**: *"Structural analysis of CAPS2 reveals conserved domains essential for membrane association"*
**作者**: Speidel D, et al.
**摘要**: 利用重组CAPS2蛋白进行结构域解析，发现其N端PH结构域和C端DCV结合域对靶向分泌囊泡至关重要。研究还通过突变实验证实这些结构域是CAPS2调控分泌功能的基础。
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以上文献均聚焦于CAPS2重组蛋白的表达、功能验证及其在分泌机制中的作用，涉及不同表达系统(细菌、昆虫细胞)和疾病关联研究。如需具体DOI或期刊信息，可进一步补充检索关键词。
CAPS2 (Calcium-dependent activator protein for secretion 2), also known as CADPS2. is a secretory granule-associated protein critical for regulating dense-core vesicle (DCV) exocytosis and neuropeptide release. It belongs to the CAPS/CADPS protein family, which shares structural homology with UNC-31 in *C. elegans*. CAPS2 is primarily expressed in neuronal and neuroendocrine cells, where it functions as a calcium-sensitive scaffolding molecule to facilitate vesicle priming, membrane fusion, and cargo secretion. Structurally, it contains a conserved N-terminal pleckstrin homology (PH) domain for membrane lipid interactions, a central calcium-binding C2 domain, and a C-terminal domain involved in vesicle association.
Research highlights its role in neurodevelopment and synaptic plasticity. CAPS2 knockout mice exhibit deficits in social behavior, synaptic transmission, and brain-derived neurotrophic factor (BDNF) secretion, linking it to autism spectrum disorders (ASD) and neuropsychiatric conditions. Human studies have identified CADPS2 gene variants associated with ASD, schizophrenia, and bipolar disorder, underscoring its clinical relevance.
Recombinant CAPS2 proteins are typically produced in *E. coli* or mammalian expression systems, enabling biochemical and functional studies. Purification often involves affinity chromatography and tag-based methods. These recombinant tools are vital for dissecting molecular mechanisms of neurosecretion, screening therapeutic compounds, and modeling disease-associated mutations. Recent advances also explore its potential in gene therapy and targeted drug delivery systems for neurological disorders. Ongoing research aims to elucidate its interplay with SNARE complexes and Rab GTPases in spatiotemporal control of exocytosis.
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