| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BEST2 |
| Uniprot No | Q8NFU1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-509aa |
| 氨基酸序列 | MTVTYTARVANARFGGFSQLLLLWRGSIYKLLWRELLCFLGFYMALSAAYRFVLTEGQKRYFEKLVIYCDQYASLIPVSFVLGFYVTLVVNRWWSQYLCMPLPDALMCVVAGTVHGRDDRGRLYRRTLMRYAGLSAVLILRSVSTAVFKRFPTIDHVVEAGFMTREERKKFENLNSSYNKYWVPCVWFSNLAAQARREGRIRDNSALKLLLEELNVFRGKCGMLFHYDWISVPLVYTQVVTIALYSYFLACLIGRQFLDPAQGYKDHDLDLCVPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNFLIDRNFQVSMLAVDEMYDDLAVLEKDLYWDAAEARAPYTAATVFQLRQPSFQGSTFDITLAKEDMQFQRLDGLDGPMGEAPGDFLQRLLPAGAGMVAGGPLGRRLSFLLRKNSCVSEASTGASCSCAVVPEGAAPECSCGDPLLDPGLPEPEAPPPAGPEPLTLIPGPVEPFSIVTMPGPRGPAPPWLPSPIGEEEENLA |
| 预测分子量 | 57,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BEST2重组蛋白的3篇参考文献示例(内容基于真实研究领域，具体作者和标题为模拟示例，建议通过学术数据库核实)：
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1. **标题**：*"Functional Analysis of Recombinant Bestrophin-2 (BEST2) Expressed in Mammalian Cells*
**作者**：Hartzell, H.C., et al.
**摘要**：研究利用HEK293细胞重组表达BEST2蛋白，通过膜片钳技术证实其钙激活氯离子通道功能，并探讨其与视网膜疾病的相关性。
2. **标题**：*"Structural Insights into BEST2 Ion Permeation by Cryo-EM*
**作者**：Yang, T., et al.
**摘要**：通过冷冻电镜解析重组BEST2蛋白的三维结构，揭示其离子选择性滤器的分子机制，为突变导致的通道功能障碍提供解释。
3. **标题**：*"BEST2 Modulates Intestinal Fluid Secretion via CFTR Interaction*
**作者**：Stanton, B.A., et al.
**摘要**：在结肠上皮细胞中重组表达BEST2.发现其通过调控CFTR通道活性影响氯离子分泌，提示其在肠道疾病中的潜在作用。
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**注意**：以上文献标题和作者为示例性质，具体文献需通过PubMed或Google Scholar以关键词“BEST2 recombinant protein”“Bestrophin-2 expression”等检索获取。真实研究可参考：
- Bakall, B. et al. (2008) 对BEST2功能的早期表征;
- Milenkovic, V.M. et al. (2011) 关于BEST2在视网膜色素上皮中的作用。
**Background of BEST2 Recombinant Protein**
BEST2 (Bestrophin-2) is a member of the bestrophin family of calcium-activated chloride channels (CaCCs), which play critical roles in regulating ion transport and cellular homeostasis. The bestrophin family comprises four isoforms (BEST1–BEST4) in humans, with BEST2 being primarily expressed in epithelial tissues, including the gastrointestinal tract, respiratory system, and pancreas. It is structurally characterized by a conserved transmembrane domain with five predicted transmembrane helices, forming a homopentameric ion channel that facilitates chloride ion flux in response to intracellular calcium signaling.
BEST2 has garnered attention for its physiological and pathological implications. Studies suggest its involvement in fluid secretion, cell volume regulation, and pH balance, particularly in secretory epithelia. Unlike BEST1. which is linked to retinal degenerative diseases (e.g., Best vitelliform macular dystrophy), BEST2 is less studied but has been implicated in gastrointestinal motility disorders and cystic fibrosis-related ion transport defects. Its role in pancreatic ductal bicarbonate secretion also highlights potential relevance in digestive and metabolic diseases.
Recombinant BEST2 protein is engineered using heterologous expression systems (e.g., mammalian cells, *E. coli*) to study its biophysical properties, channel gating mechanisms, and interactions with regulatory proteins. This protein serves as a tool for structure-function analyses, drug screening, and understanding CaCC biology. Recent advances in cryo-EM have enabled high-resolution structural insights, aiding the design of modulators targeting chloride channelopathies.
Despite progress, functional redundancy among bestrophins and limited tissue-specific data pose challenges. Ongoing research aims to elucidate BEST2’s physiological roles and therapeutic potential, particularly in epithelial ion transport disorders.
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