| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | USP2 |
| Uniprot No | O75604 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-605aa |
| 氨基酸序列 | MDYKDDDDKSQLSSTLKRYTESARYTDAHYAKSGYGAYTPSSYGANLAAS LLEKEKLGFKPVPTSSFLTRPRTYGPSSLLDYDRGRPLLRPDITGGGKRA ESQTRGTERPLGSGLSGGSGFPYGVTNNCLSYLPINAYDQGVTLTQKLDS QSDLARDFSSLRTSDSYRIDPRNLGRSPMLARTRKELCTLQGLYQTASCP EYLVDYLENYGRKGSASQVPSQAPPSRVPEIISPTYRPIGRYTLWETGKG QAPGPSRSSSPGRDGMNSKSAQGLAGLRNLGNTCFMNSILQCLSNTRELR DYCLQRLYMRDLHHGSNAHTALVEEFAKLIQTIWTSSPNDVVSPSEFKTQ IQRYAPRFVGYNQQDAQEFLRFLLDGLHNEVNRVTLRPKSNPENLDHLPD DEKGRQMWRKYLEREDSRIGDLFVGQLKSSLTCTDCGYCSTVFDPFWDLS LPIAKRGYPEVTLMDCMRLFTKEDVLDGDEKPTCCRCRGRKRCIKKFSIQ RFPKILVLHLKRFSESRIRTSKLTTFVNFPLRDLDLREFASENTNHAVYN LYAVSNHSGTTMGGHYTAYCRSPGTGEWHTFNDSSVTPMSSSQVRTSDAY LLFYELASPPSRM |
| 预测分子量 | 68 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于USP2重组蛋白的3篇参考文献示例(内容基于公开研究概括，非真实文献)：
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1. **文献名称**：Structural insights into USP2 activation by ubiquitin and phosphorylation
**作者**：Smith A, et al.
**摘要**：通过重组表达人源USP2催化结构域，解析其晶体结构，揭示泛素结合诱导的构象变化及磷酸化修饰对去泛素化酶活性的调控机制。
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2. **文献名称**：Recombinant USP2 regulates the stabilization of p53 via deubiquitination in cancer cells
**作者**：Chen L, et al.
**摘要**：利用大肠杆菌表达纯化的USP2重组蛋白，证明其通过去泛素化抑制p53降解，增强肿瘤细胞中p53稳定性并诱导凋亡，提示其作为抗癌靶点的潜力。
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3. **文献名称**：Functional characterization of USP2 isoforms in cytokine signaling using recombinant proteins
**作者**：Wang Y, et al.
**摘要**：通过昆虫细胞系统表达USP2两种剪接变体重组蛋白，发现USP2a特异性调控NF-κB信号通路的去泛素化过程，而USP2b主要影响TGF-β通路。
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(注：以上为模拟内容，实际文献需通过学术数据库查询。)
USP2 (Ubiquitin-Specific Protease 2) is a deubiquitinating enzyme belonging to the ubiquitin-specific protease family, which plays a critical role in regulating protein stability and function by removing ubiquitin chains from substrate proteins. It is involved in diverse cellular processes, including cell cycle progression, apoptosis, DNA repair, and immune response modulation. USP2 primarily cleaves lysine-48- and lysine-63-linked polyubiquitin chains, thereby rescuing target proteins from proteasomal degradation or altering their signaling activity. Known substrates include oncoproteins (e.g., cyclin D1. MDM2) and signaling regulators (e.g., TRAF6. β-catenin), linking USP2 to cancer, inflammation, and metabolic disorders.
Recombinant USP2 protein is engineered for in vitro studies to dissect its enzymatic mechanisms, substrate specificity, and interactions. Typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, the recombinant protein retains catalytic activity when purified, often with tags (e.g., His, GST) for detection and affinity purification. Researchers use it to screen small-molecule inhibitors, characterize structural domains (e.g., catalytic cleft mutations), and analyze deubiquitination kinetics. Its role in stabilizing oncoproteins like fatty acid synthase (FASN) and androgen receptor (AR) has made USP2 a therapeutic target in cancers, particularly prostate cancer. Additionally, USP2’s involvement in circadian rhythm regulation and lipid metabolism highlights its broader physiological relevance. Studies with recombinant USP2 have advanced understanding of ubiquitin-dependent pathways and spurred drug discovery efforts targeting deubiquitinases.
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