| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | XPO4 |
| Uniprot No | Q9C0E2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1151aa |
| 氨基酸序列 | MMAAALGPPEVIAQLENAAKVLMAPPSMVNNEQRQHAEHIFLSFRKSKSPFAVCKHILETSKVDYVLFQAATAIMEAVVREWILLEKGSIESLRTFLLTYVLQRPNLQKYVREQILLAVAVIVKRGSLDKSIDCKSIFHEVSQLISSGNPTVQTLACSILTALLSEFSSSSKTSNIGLSMEFHGNCKRVFQEEDLRQIFMLTVEVLQEFSRRENLNAQMSSVFQRYLALANQVLSWNFLPPNLGRHYIAMFESSQNVLLKPTESWRETLLDSRVMELFFTVHRKIREDSDMAQDSLQCLAQLASLHGPIFPDEGSQVDYLAHFIEGLLNTINGIEIEDSEAVGISSIISNLITVFPRNVLTAIPSELFSSFVNCLTHLTCSFGRSAALEEVLDKDDMVYMEAYDKLLESWLTLVQDDKHFHKGFFTQHAVQVFNSYIQCHLAAPDGTRNLTANGVASREEEEISELQEDDRDQFSDQLASVGMLGRIAAEHCIPLLTSLLEERVTRLHGQLQRHQQQLLASPGSSTVDNKMLDDLYEDIHWLILVTGYLLADDTQGETPLIPPEIMEYSIKHSSEVDINTTLQILGSPGEKASSIPGYNRTDSVIRLLSAILRVSEVESRAIRADLTHLLSPQMGKDIVWFLKRWAKTYLLVDEKLYDQISLPFSTAFGADTEGSQWIIGYLLQKVISNLSVWSSEQDLANDTVQLLVTLVERRERANLVIQCENWWNLAKQFASRSPPLNFLSSPVQRTLMKALVLGGFAHMDTETKQQYWTEVLQPLQQRFLRVINQENFQQMCQQEEVKQEITATLEALCGIAEATQIDNVAILFNFLMDFLTNCIGLMEVYKNTPETVNLIIEVFVEVAHKQICYLGESKAMNLYEACLTLLQVYSKNNLGRQRIDVTAEEEQYQDLLLIMELLTNLLSKEFIDFSDTDEVFRGHEPGQAANRSVSAADVVLYGVNLILPLMSQDLLKFPTLCNQYYKLITFICEIFPEKIPQLPEDLFKSLMYSLELGMTSMSSEVCQLCLEALTPLAEQCAKAQETDSPLFLATRHFLKLVFDMLVLQKHNTEMTTAAGEAFYTLVCLHQAEYSELVETLLSSQQDPVIYQRLADAFNKLTASSTPPTLDRKQKMAFLKSLEEFMANVGGLLCVK |
| 预测分子量 | 130 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于XPO4(Exportin-4)重组蛋白的模拟参考文献示例(注：部分信息为假设性概括，实际文献需通过学术数据库验证)：
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1. **文献名称**: *Structural basis for the recognition of phosphorylated adaptor proteins by Exportin-4*
**作者**: Güttler, T., et al.
**摘要**: 本研究解析了XPO4重组蛋白与磷酸化底物(如SMAD3)的复合物晶体结构，揭示了XPO4通过特异性识别磷酸化基序介导核质转运的分子机制，并证实其在TGF-β信号通路中的调控作用。
2. **文献名称**: *Exportin-4 coordinates nuclear transport of the translation initiation factor eIF5A*
**作者**: Lipowsky, G., et al.
**摘要**: 通过体外重组XPO4蛋白实验，证明其与真核翻译起始因子eIF5A的相互作用依赖于RanGTP，阐明了XPO4在调控蛋白质合成及细胞周期进程中的关键功能。
3. **文献名称**: *XPO4 depletion promotes epithelial-mesenchymal transition via TGF-β signaling in cancer cells*
**作者**: Bahlis, K., et al.
**摘要**: 利用重组XPO4蛋白进行功能拯救实验，发现XPO4通过输出SMAD转录因子抑制TGF-β介导的肿瘤细胞上皮-间质转化(EMT)，为癌症转移机制提供了新视角。
4. **文献名称**: *Recombinant Exportin-4 characterization and its role in viral RNA export*
**作者**: Fung, H.Y., et al.
**摘要**: 报道了XPO4重组蛋白的高效表达与纯化方法，并发现其参与特定病毒RNA的核输出过程，提示XPO4在宿主抗病毒免疫反应中的潜在作用。
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建议通过PubMed或Google Scholar以“XPO4 recombinant protein”“Exportin-4 structure/function”等关键词检索真实文献。
XPO4 (Exportin-4) is a member of the karyopherin-β family of nuclear transport receptors, primarily responsible for mediating the export of specific protein cargoes from the nucleus to the cytoplasm. It plays a critical role in maintaining cellular homeostasis by regulating the nucleocytoplasmic shuttling of transcription factors, tumor suppressors, and RNA-binding proteins. Unlike other exportins such as XPO1/CRM1. XPO4 specifically recognizes cargoes bearing a unique nuclear export signal (NES), including SMAD transcription factors (e.g., SMAD2/3) and SOX family proteins, which are pivotal in TGF-β signaling, embryonic development, and cancer progression.
The recombinant XPO4 protein is engineered using expression systems like *E. coli* or mammalian cells, enabling large-scale production of functional, purified XPO4 for research and therapeutic applications. Its recombinant form retains the ability to bind cargoes and RanGTP, a small GTPase essential for the transport cycle. Structural studies reveal that XPO4 contains characteristic HEAT repeats, facilitating interactions with cargoes and regulatory partners.
Dysregulation of XPO4 is implicated in diseases such as cancer, fibrosis, and neurodegenerative disorders. For instance, aberrant XPO4 activity may disrupt SMAD localization, contributing to TGF-β-driven metastasis or fibrosis. Conversely, XPO4 can act as a tumor suppressor by exporting oncogenic proteins. This dual role underscores its context-dependent functionality.
Recombinant XPO4 is widely used to study nuclear transport mechanisms, screen for small-molecule inhibitors, and develop targeted therapies. Recent efforts focus on modulating XPO4 to restore normal protein trafficking in pathological conditions. However, its therapeutic potential remains under exploration compared to XPO1. highlighting the need for further mechanistic and clinical studies.
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