| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCOR1 |
| Uniprot No | O75376 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-373aa |
| 氨基酸序列 | MSSSGYPPNQGAFSTEQSRYPPHSVQYTFPNTRHQQEFAVPDYRSSHLEVSQASQLLQQQQQQQLRRRPSLLSEFHPGSDRPQERRTSYEPFHPGPSPVDHDSLESKRPRLEQVSDSHFQRVSAAVLPLVHPLPEGLRASADAKKDPAFGGKHEAPSSPISGQPCGDDQNASPSKLSKEELIQSMDRVDREIAKVEQQILKLKKKQQQLEEEAAKPPEPEKPVSPPPVEQKHRSIVQIIYDENRKKAEEAHKIFEGLGPKVELPLYNQPSDTKVYHENIKTNQVMRKKLILFFKRRNHARKQREQKICQRYDQLMEAWEKKVDRIENNPRRKAKESKTREYYEKQFPEIRKQREQQERFQRVGQRGAGLSATI |
| 预测分子量 | 48.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NCOR1重组蛋白的3篇代表性文献的简要信息(注：文献为示例性概括，实际引用时需核实来源)：
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1. **文献名称**：*Structural and functional analysis of the NCOR1 repressor domain in nuclear receptor signaling*
**作者**：Watson, L.C., et al.
**摘要**：该研究通过重组表达并纯化NCOR1蛋白的抑制结构域，结合X射线晶体学解析其三维结构，揭示了其与核受体(如甲状腺激素受体)相互作用的分子机制，并验证了关键氨基酸突变对转录抑制功能的影响。
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2. **文献名称**：*NCOR1/SMRT complex recruitment by agonist-bound estrogen receptor-alpha*
**作者**：Hu, X., et al.
**摘要**：利用重组NCOR1蛋白和体外互作实验，证明雌激素受体α(ERα)在配体结合状态下通过特定构象变化招募NCOR1/SMRT共抑制复合物，揭示了受体动态调控基因沉默的分子基础。
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3. **文献名称**：*Recombinant NCOR1 protein rescues transcriptional repression defects in a mouse model of neurological disorders*
**作者**：Yamamoto, H., et al.
**摘要**：通过体外重组NCOR1蛋白递送至NCOR1缺失的神经细胞，恢复了组蛋白去乙酰化酶(HDAC3)的募集能力，改善了小鼠模型中神经元基因表达失调和相关行为异常，为靶向共抑制因子的治疗策略提供依据。
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**建议**：以上文献为示例，实际研究中可通过PubMed或Google Scholar以关键词“NCOR1 recombinant protein”、“NCoR1 purification”等检索最新文献，重点关注蛋白纯化方法、结构功能研究或疾病相关机制方向。
The nuclear receptor co-repressor 1 (NCOR1) is a critical transcriptional regulatory protein involved in gene silencing and chromatin remodeling. As a core component of multi-protein co-repressor complexes, NCOR1 mediates the repression of target genes by recruiting histone deacetylases (HDACs) and other epigenetic modifiers to nuclear receptors and transcription factors. It plays a pivotal role in diverse physiological processes, including development, metabolism, and immune response, by fine-tuning hormone signaling pathways such as those regulated by thyroid hormone receptors, estrogen receptors, and retinoic acid receptors. Dysregulation of NCOR1 has been linked to cancers, metabolic syndromes, and neurodevelopmental disorders, highlighting its therapeutic and diagnostic potential.
Recombinant NCOR1 proteins are engineered to study its molecular interactions and functions in vitro or in cellular models. These proteins are typically produced using expression systems like Escherichia coli or mammalian cells, ensuring proper post-translational modifications for functional studies. Purified recombinant NCOR1 enables researchers to analyze its structural domains, binding partners (e.g., HDAC3. SMRT), and mechanisms of gene repression through biochemical assays, X-ray crystallography, or cryo-EM. It also serves as a tool for screening small molecules that modulate NCOR1-dependent pathways, offering insights into drug development for hormone-resistant cancers or metabolic diseases. Recent studies further explore its role in circadian rhythm regulation and inflammatory responses, expanding its relevance in precision medicine.
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