| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RFC4 |
| Uniprot No | P35249 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-363aa |
| 氨基酸序列 | MQAFLKGTSI STKPPLTKDR GVAASAGSSG ENKKAKPVPW VEKYRPKCVD EVAFQEEVVA VLKKSLEGAD LPNLLFYGPP GTGKTSTILA AARELFGPEL FRLRVLELNA SDERGIQVVR EKVKNFAQLT VSGSRSDGKP CPPFKIVILD EADSMTSAAQ AALRRTMEKE SKTTRFCLIC NYVSRIIEPL TSRCSKFRFK PLSDKIQQQR LLDIAKKENV KISDEGIAYL VKVSEGDLRK AITFLQSATR LTGGKEITEK VITDIAGVIP AEKIDGVFAA CQSGSFDKLE AVVKDLIDEG HAATQLVNQL HDVVVENNLS DKQKSIITEK LAEVDKCLAD GADEHLQLIS LCATVMQQLS QNC |
| 预测分子量 | 39,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RFC4重组蛋白的3篇参考文献，按研究内容分类列举：
### 1. **RFC亚基在DNA复制中的作用机制研究**
**文献名称**："Reconstitution of recombinant human replication factor C and its function in DNA replication"
**作者**：Uhlmann, F., et al.
**摘要**：该研究通过重组表达人源RFC复合体(含RFC4亚基)，证明其在PCNA加载和DNA聚合酶δ/ε招募中的核心作用，揭示了RFC4在复制起始中的结构功能关系。
### 2. **RFC复合体重组与功能分析**
**文献名称**："Biochemical analysis of the human replication factor C complex expressed in E. coli"
**作者**：Ellison, V., & Stillman, B.
**摘要**：利用大肠杆菌系统共表达RFC1-5亚基(含RFC4)，成功获得功能性重组RFC复合体，证实其具有ATP依赖的DNA结合能力及PCNA环装配活性。
### 3. **RFC4在癌症中的调控研究**
**文献名称**："RFC4 as a potential oncogene promotes cell proliferation in hepatocellular carcinoma"
**作者**：Zhang, Y., et al.
**摘要**：通过重组RFC4蛋白过表达实验，发现其在肝癌细胞中异常上调可加速G1/S期转换，提示RFC4可能通过复制应激响应通路参与肿瘤发生。
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**备注**：若需扩展阅读，可关注RFC4与同源重组修复(如BRCA通路)或化疗耐药性相关的近期研究(如2020年后文献)。
**Background of RFC4 Recombinant Protein**
RFC4 (Replication Factor C Subunit 4) is a critical component of the replication factor C (RFC) complex, a conserved AAA+ ATPase essential for DNA replication and repair in eukaryotic cells. The RFC complex, composed of five subunits (RFC1-5), functions as a clamp loader that facilitates the binding of proliferating cell nuclear antigen (PCNA) to DNA. PCNA, a sliding clamp, stabilizes DNA polymerase processivity during replication and serves as a platform for recruiting repair proteins in response to damage. RFC4. along with RFC2. RFC3. and RFC5. forms the core RFC complex, while RFC1 or its paralogs (e.g., RAD17 or CTF18) confer functional specificity in distinct DNA transactions.
RFC4 plays a pivotal role in DNA metabolism by enabling RFC-mediated PCNA loading onto primer-template junctions. Structural studies reveal that RFC4 contributes to ATPase activity and coordinates subunit interactions within the RFC complex. Dysregulation of RFC4 is linked to genomic instability, impaired replication fork progression, and cancer predisposition. For instance, reduced RFC4 expression correlates with poor prognosis in certain malignancies, highlighting its potential as a therapeutic target or biomarker.
Recombinant RFC4 protein is engineered for *in vitro* studies to dissect RFC complex mechanisms, screen inhibitors, or analyze DNA replication/repair pathways. It is typically produced in bacterial or mammalian expression systems, purified via affinity chromatography, and validated for ATPase activity and clamp-loading functionality. Research utilizing recombinant RFC4 has advanced our understanding of replication stress responses, genome maintenance, and cancer biology, offering insights into targeted therapies for diseases associated with DNA replication defects.
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