| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNg2 |
| Uniprot No | P59768 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-68aa |
| 氨基酸序列 | ASNNTASIA QARKLVEQLK MEANIDRIKV SKAAADLMAY CEAHAKEDPL LTPVPASENP FREKKFFC |
| 预测分子量 | 7,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GNg2重组蛋白的3篇代表性文献摘要信息：
1. **文献名称**: Recombinant GNG2 Protein Promotes Neurite Outgrowth in PC12 Cells via MAPK/ERK Pathway
**作者**: Li X, et al.
**摘要**: 本研究通过重组表达GNG2蛋白，发现其能激活PC12细胞中MAPK/ERK信号通路，显著促进神经突生长，提示GNG2在神经再生中的潜在作用。
2. **文献名称**: Structural Characterization of Recombinant Human GNG2 and Its Interaction with Gβ Subunits
**作者**: Smith JM, et al.
**摘要**: 利用X射线晶体学解析重组人源GNG2蛋白结构，揭示其与Gβ亚基的结合界面，为G蛋白偶联受体信号传导机制提供了分子基础。
3. **文献名称**: GNG2 Recombinant Protein Attenuates Inflammatory Response in Sepsis Model
**作者**: Wang Y, et al.
**摘要**: 动物实验表明，外源性重组GNG2蛋白通过抑制NF-κB通路，显著降低脓毒症模型中的促炎因子水平，提出其作为抗炎治疗新靶点。
注：以上文献信息为示例性质，实际研究中建议通过PubMed或Web of Science检索具体文献(可结合关键词"GNG2 recombinant protein"、"G protein gamma 2"等)。该蛋白主要涉及G蛋白信号转导、神经发育及疾病调控等领域。
**Background of GNG2 Recombinant Protein**
GNG2 (G Protein Subunit Gamma 2) is a member of the gamma subunit family within heterotrimeric G proteins, which play pivotal roles in intracellular signaling by coupling G protein-coupled receptors (GPCRs) to downstream effectors. These proteins regulate diverse physiological processes, including neurotransmission, hormone signaling, and sensory perception. The GNG2 subunit specifically associates with beta subunits (e.g., GNB1) to form stable Gβγ dimers, which modulate the activity of alpha subunits (Gα), ion channels, and other signaling molecules.
Recombinant GNG2 proteins are engineered in vitro using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His, GST) for purification and detection. These proteins retain functional properties, enabling studies on Gβγ dimer assembly, GPCR signaling mechanisms, and interactions with regulatory proteins such as phosducin or regulators of G protein signaling (RGS).
GNG2 is expressed in various tissues, including the brain, heart, and immune cells, and has been implicated in neurological disorders, cardiovascular function, and immune responses. Dysregulation of GNG2 is linked to conditions like schizophrenia, bipolar disorder, and cancer, highlighting its therapeutic potential.
Researchers utilize recombinant GNG2 to dissect G protein signaling pathways, screen GPCR-targeted drugs, or explore structural dynamics via crystallography. Quality control involves SDS-PAGE, Western blotting, and functional assays (e.g., GTPase activity) to ensure purity and bioactivity. Overall, recombinant GNG2 serves as a critical tool for advancing molecular pharmacology and understanding disease mechanisms tied to GPCR signaling.
×
