| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NARF |
| Uniprot No | Q9UHQ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-456aa |
| 氨基酸序列 | MKCEHCTRKE CSKKTKTDDQ ENVSADAPSP AQENGEKGEF HKLADAKIFL SDCLACDSCM TAEEGVQLSQ QNAKDFFRVL NLNKKCDTSK HKVLVVSVCP QSLPYFAAKF NLSVTDASRR LCGFLKSLGV HYVFDTTIAA DFSILESQKE FVRRYRQHSE EERTLPMLTS ACPGWVRYAE RVLGRPITAH LCTAKSPQQV MGSLVKDYFA RQQNLSPEKI FHVIVAPCYD KKLEALQESL PPALHGSRGA DCVLTSGEIA QIMEQGDLSV RDAAVDTLFG DLKEDKVTRH DGASSDGHLA HIFRHAAKEL FNEDVEEVTY RALRNKDFQE VTLEKNGEVV LRFAAAYGFR NIQNMILKLK KGKFPFHFVE VLACAGGCLN GRGQAQTPDG HADKALLRQM EGIYADIPVR RPESSAHVQE LYQEWLEGIN SPKAREVLHT TYQSQERGTH SLDIKW |
| 预测分子量 | 51,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NARF重组蛋白的3条参考文献示例(注：NARF相关研究较为小众，以下内容为模拟虚构文献，仅供参考)：
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1. **文献名称**：*NARF Recombinant Protein Facilitates Ribosome Biogenesis in Arabidopsis*
**作者**：Chen, L. et al.
**摘要**：研究利用重组NARF蛋白揭示了其在拟南芥核糖体RNA加工中的作用，表明NARF通过结合特定RNA结构域调控核仁组装与胁迫响应。
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2. **文献名称**：*Expression and Functional Analysis of NARF in DNA Damage Repair*
**作者**：Gomez, R. et al.
**摘要**：通过大肠杆菌表达系统获得高纯度NARF重组蛋白，实验证实其与人类DNA损伤修复复合体相互作用，并增强细胞对电离辐射的抗性。
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3. **文献名称**：*Crystal Structure of NARF Reveals a Unique DNA-Binding Motif*
**作者**：Tanaka, K. et al.
**摘要**：解析了NARF重组蛋白的晶体结构，发现其新型DNA结合域对端粒稳定性至关重要，为靶向癌症治疗提供潜在分子基础。
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**提示**：若需真实文献，建议核查"NARF"是否为特定领域术语(如植物学中的核仁相关因子)，或确认名称拼写(如与"Nrf2"区分)。可通过PubMed或Web of Science以“NARF + recombinant protein”为关键词进一步检索。
NARF (Novel Autophagy-Regulating Factor) recombinant protein is a engineered biomolecule designed to study or modulate autophagy, a critical cellular process responsible for degrading and recycling damaged organelles, misfolded proteins, and pathogens. Autophagy dysregulation is implicated in various diseases, including neurodegenerative disorders (e.g., Alzheimer’s, Parkinson’s), cancer, and metabolic syndromes. NARF was identified through genomic or proteomic screens as a protein interacting with core autophagy machinery, such as ATG proteins or LC3 homologs, though its exact molecular mechanism remains under investigation.
Recombinant NARF is typically produced using expression systems like *E. coli* or mammalian cell cultures, followed by purification via affinity tags (e.g., His-tag) and chromatography. Its recombinant form enables precise control in experimental settings, allowing researchers to explore its role in autophagy initiation, autophagosome formation, or lysosomal fusion. Studies suggest NARF may act as a scaffold protein, facilitating interactions between autophagy-related complexes, or as a post-translational modifier influencing pathway activation.
Applications include in vitro assays to dissect autophagy signaling, drug discovery screens targeting autophagy-related diseases, and functional studies in cell or animal models. For instance, NARF overexpression or knockdown experiments help clarify its impact on cellular survival under stress (e.g., nutrient deprivation). Challenges include optimizing protein stability and activity in diverse biological systems. Ongoing research aims to characterize its structure-function relationships and therapeutic potential, positioning NARF recombinant protein as a valuable tool in autophagy and disease biology.
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