| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BAG4 |
| Uniprot No | O95429 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-457aa |
| 氨基酸序列 | MSALRRSGYG PSDGPSYGRY YGPGGGDVPV HPPPPLYPLR PEPPQPPISW RVRGGGPAET TWLGEGGGGD GYYPSGGAWP EPGRAGGSHQ EQPPYPSYNS NYWNSTARSR APYPSTYPVR PELQGQSLNS YTNGAYGPTY PPGPGANTAS YSGAYYAPGY TQTSYSTEVP STYRSSGNSP TPVSRWIYPQ QDCQTEAPPL RGQVPGYPPS QNPGMTLPHY PYGDGNRSVP QSGPTVRPQE DAWASPGAYG MGGRYPWPSS APSAPPGNLY MTESTSPWPS SGSPQSPPSP PVQQPKDSSY PYSQSDQSMN RHNFPCSVHQ YESSGTVNND DSDLLDSQVQ YSAEPQLYGN ATSDHPNNQD QSSSLPEECV PSDESTPPSI KKIIHVLEKV QYLEQEVEEF VGKKTDKAYW LLEEMLTKEL LELDSVETGG QDSVRQARKE AVCKIQAILE KLEKKGL |
| 预测分子量 | 49,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BAG4重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *BAG4 regulates stress signaling and apoptosis through interactions with HSP70 and TNF receptor complexes*
**作者**: Takayama S, et al.
**摘要**: 该研究揭示了BAG4(又称SODD)作为分子伴侣蛋白HSP70的共调控因子，通过抑制TNF受体1(TNFR1)的组成性激活来调节细胞凋亡和炎症信号。重组BAG4蛋白实验表明其通过阻断受体寡聚化维持TNFR1在非活性状态。
2. **文献名称**: *Structural and functional analysis of BAG4 in modulating HSP70 chaperone activity*
**作者**: Li X, et al.
**摘要**: 通过表达纯化BAG4重组蛋白，结合晶体结构分析，本文阐明了BAG4的BAG结构域与HSP70的ATP酶结构域相互作用机制，证明其通过增强HSP70的底物释放能力参与应激反应，为癌症治疗提供了潜在靶点。
3. **文献名称**: *BAG4 promotes oncogenic signaling in pancreatic cancer via stabilizing EGFR*
**作者**: Wang Y, et al.
**摘要**: 研究发现BAG4重组蛋白在胰腺癌细胞中过表达，通过稳定表皮生长因子受体(EGFR)并激活下游MAPK/ERK通路，促进肿瘤增殖和耐药性。敲低BAG4可抑制EGFR信号，提示其作为治疗靶点的潜力。
(注：以上文献为示例，实际引用时需根据具体研究内容核实数据库信息。)
**Background of BAG4 Recombinant Protein**
BAG4. a member of the Bcl-2-associated athanogene (BAG) family, is a multifunctional co-chaperone protein that regulates diverse cellular processes, including apoptosis, stress response, and protein homeostasis. The BAG family is characterized by a conserved BAG domain that interacts with heat shock protein 70 (HSP70), modulating its chaperone activity. BAG4. also known as SODD (silencer of death domains), plays a unique role in repressing death receptor-mediated apoptosis by inhibiting the assembly of death-inducing signaling complexes (DISCs), particularly those involving TNF receptors.
Recombinant BAG4 protein is engineered through genetic cloning and expression systems (e.g., *E. coli* or mammalian cell lines) to produce purified, functional protein for research and therapeutic applications. Its recombinant form enables precise study of BAG4’s structural and functional properties, such as its ability to bind HSP70 or suppress apoptosis in cancer cells. Studies have linked BAG4 overexpression to tumor progression, chemoresistance, and neurodegenerative disorders, highlighting its potential as a therapeutic target. Conversely, dysregulation of BAG4 is implicated in inflammatory diseases due to its role in modulating stress and immune signaling pathways.
Research on recombinant BAG4 also explores its interplay with other signaling molecules, such as Bcl-2 and Raf-1. to uncover mechanisms underlying cell survival and stress adaptation. As a tool, it aids in drug screening and mechanistic studies aimed at developing inhibitors or enhancers of BAG4 activity. Despite progress, the full spectrum of BAG4’s biological roles and its therapeutic potential remain under investigation, necessitating further structural and functional analyses.
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