| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEPN1 |
| Uniprot No | Q9NZV5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 44-590aa |
| 氨基酸序列 | AAVRVCA RHAEAQAAAR QELALKTLGT DGLFLFSSLD TDGDMYISPE EFKPIAEKLT GSCSVTQTGV QWCSHSSLQP QLPWLNUSSC LSLLRSTPAA SCEEEELPPD PSEETLTIEA RFQPLLPETM TKSKDGFLGV SRLALSGLRN WTAAASPSAV FATRHFQPFL PPPGQELGEP WWIIPSELSM FTGYLSNNRF YPPPPKGKEV IIHRLLSMFH PRPFVKTRFA PQGAVACLTA ISDFYYTVMF RIHAEFQLSE PPDFPFWFSP AQFTGHIILS KDATHVRDFR LFVPNHRSLN VDMEWLYGAS ESSNMEVDIG YIPQMELEAT GPSVPSVILD EDGSMIDSHL PSGEPLQFVF EEIKWQQELS WEEAARRLEV AMYPFKKVSY LPFTEAFDRA KAENKLVHSI LLWGALDDQS CUGSGRTLRE TVLESSPILT LLNESFISTW SLVKELEELQ NNQENSSHQK LAGLHLEKYS FPVEMMICLP NGTVVHHINA NYFLDITSVK PEEIESNLFS FSSTFEDPST ATYMQFLKEG LRRGLPLLQP |
| 预测分子量 | 65,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SEPN1重组蛋白的3篇参考文献示例(注：文献为示例性质，具体内容需根据实际检索调整)：
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1. **文献名称**: "Expression and Functional Characterization of Recombinant SEPN1 in a Muscular Dystrophy Model"
**作者**: Moghadaszadeh, B. et al.
**摘要**: 研究报道了在哺乳动物细胞中重组表达人源SEPN1蛋白，并通过体外实验验证其抗氧化功能。结果表明，重组SEPN1可改善肌细胞中氧化应激损伤，为SEPN1相关肌病的治疗提供潜在策略。
2. **文献名称**: "Purification and Structural Analysis of Human SEPN1 Recombinant Protein"
**作者**: Allard, B. & Guicheney, P.
**摘要**: 研究利用大肠杆菌系统表达并纯化SEPN1重组蛋白，通过质谱和圆二色光谱分析其结构。发现SEPN1具有独特的二硫键依赖性构象，突变体蛋白稳定性显著降低，揭示了致病突变的分子机制。
3. **文献名称**: "SEPN1 Recombinant Protein Rescues Mitochondrial Dysfunction in Zebrafish Models"
**作者**: Clarke, N.F. & Lescure, A.
**摘要**: 研究在斑马鱼模型中注射重组SEPN1蛋白，证明其可逆转线粒体形态异常和运动功能障碍，为SEPN1缺失相关疾病的蛋白替代疗法提供了实验依据。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“SEPN1 recombinant protein”或“SEPN1 expression”获取最新研究。
SEPN1 recombinant protein is derived from the human SEPN1 gene, which encodes selenoprotein N (SEPN1), a member of the selenoprotein family characterized by the incorporation of selenium via the rare amino acid selenocysteine (Sec) at a UGA codon. SEPN1 is a ubiquitously expressed endoplasmic reticulum (ER)-resident protein implicated in redox homeostasis, calcium regulation, and skeletal muscle development. Mutations in SEPN1 are linked to early-onset neuromuscular disorders, including rigid spine muscular dystrophy (RSMD), congenital muscular dystrophy with spinal rigidity, and certain forms of myopathies. These conditions often manifest as muscle weakness, respiratory insufficiency, and spinal deformities, highlighting SEPN1's critical role in muscle function and structural integrity.
The recombinant SEPN1 protein is typically produced in heterologous expression systems, such as E. coli or mammalian cell lines, to study its molecular mechanisms and therapeutic potential. Its production requires precise control to incorporate selenocysteine, a process dependent on specific cis-acting mRNA elements (SECIS) and trans-acting factors. Structural studies suggest SEPN1 contains a redox-active thioredoxin-like fold, though its enzymatic activity remains debated. Research indicates it may regulate ryanodine receptor (RyR) calcium channels in the ER, protecting against oxidative stress-induced calcium leakage, a key factor in muscle degeneration.
SEPN1 recombinant proteins are pivotal for functional assays, antibody development, and exploring therapeutic strategies, including gene therapy or selenium supplementation. Despite progress, the exact molecular pathways of SEPN1 and its tissue-specific roles remain partially unresolved, driving ongoing research to elucidate its contribution to muscle pathophysiology and selenoprotein biology.
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