| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDC27 |
| Uniprot No | P30260 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-824aa |
| 氨基酸序列 | MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF |
| 预测分子量 | 91,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDC27重组蛋白的模拟参考文献示例(注：文献信息为假设性概括，建议通过学术数据库核实具体内容)：
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1. **文献名称**：*Structural Insights into the Human APC/C-CDC27 Subunit Revealed by Recombinant Protein Expression*
**作者**：Barford D., et al.
**摘要**：本研究通过重组表达技术纯化人源CDC27蛋白，结合冷冻电镜解析了其作为APC/C复合体核心亚基的三维结构，揭示了其与底物识别相关的保守结构域及磷酸化修饰位点。
2. **文献名称**：*Functional Analysis of CDC27 in Mitotic Exit via Recombinant Mutagenesis*
**作者**：King R.W., et al.
**摘要**：利用重组CDC27蛋白进行体外泛素化实验，证明其在APC/C介导的周期蛋白B降解中起关键作用，并发现特定突变会导致有丝分裂进程异常。
3. **文献名称**：*CDC27 Recombinant Protein Interacts with Checkpoint Regulators in DNA Damage Response*
**作者**：Zhang Y., et al.
**摘要**：通过重组CDC27与细胞周期检查点蛋白(如BUBR1)的互作实验，阐明了其在DNA损伤后调控细胞周期停滞的分子机制。
4. **文献名称**：*Expression and Purification of Active CDC27 for High-Throughput Drug Screening*
**作者**：Hershko A., et al.
**摘要**：开发了一种高效重组表达系统获得功能性CDC27蛋白，并用于筛选靶向APC/C的小分子抑制剂，为癌症治疗提供潜在策略。
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建议通过 **PubMed** 或 **Google Scholar** 检索关键词“CDC27 recombinant protein”“APC/C CDC27”等获取真实文献。
**Background of CDC27 Recombinant Protein**
CDC27. a key component of the Anaphase-Promoting Complex/Cyclosome (APC/C), plays a critical role in regulating cell cycle progression. The APC/C is a large E3 ubiquitin ligase responsible for targeting specific proteins for proteasomal degradation, thereby driving transitions between cell cycle phases. CDC27. as one of its core subunits, contributes to the structural integrity and substrate recognition of the APC/C. It is particularly essential during mitosis, where it facilitates the degradation of mitotic cyclins and securins, ensuring proper chromosome segregation and exit from mitosis. Dysregulation of CDC27 or the APC/C has been linked to genomic instability, developmental defects, and cancer.
Recombinant CDC27 protein is engineered using heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to enable biochemical and functional studies. Its production typically involves cloning the *CDC27* gene into expression vectors, followed by purification via affinity tags (e.g., His-tag). Recombinant CDC27 retains the ability to interact with other APC/C subunits, making it valuable for *in vitro* reconstitution assays, enzymatic activity studies, and probing mechanisms of ubiquitination.
Research on recombinant CDC27 has advanced understanding of APC/C assembly, substrate specificity, and regulation by coactivators like CDC20 or CDH1. It also aids in exploring mutations or post-translational modifications (e.g., phosphorylation) that impact APC/C function. Additionally, recombinant CDC27 serves as a tool for drug screening, particularly in identifying compounds that modulate APC/C activity for potential cancer therapies. Its study remains pivotal in unraveling cell cycle control mechanisms and their implications in disease.
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