| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP6C |
| Uniprot No | O00743 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-305aa |
| 氨基酸序列 | MAPLDLDKYV EIARLCKYLP ENDLKRLCDY VCDLLLEESN VQPVSTPVTV CGDIHGQFYD LCELFRTGGQ VPDTNYIFMG DFVDRGYYSL ETFTYLLALK AKWPDRITLL RGNHESRQIT QVYGFYDECQ TKYGNANAWR YCTKVFDMLT VAALIDEQIL CVHGGLSPDI KTLDQIRTIE RNQEIPHKGA FCDLVWSDPE DVDTWAISPR GAGWLFGAKV TNEFVHINNL KLICRAHQLV HEGYKFMFDE KLVTVWSAPN YCYRCGNIAS IMVFKDVNTR EPKLFRAVPD SERVIPPRTT TPYFL |
| 预测分子量 | 35,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP6C重组蛋白的3篇参考文献示例(注：以下文献信息为示例性质，实际引用时请核实原文)：
1. **文献名称**：*"Expression and Functional Characterization of Recombinant PPP6C Catalytic Subunit in Human Cells"*
**作者**：Chen et al.
**摘要**：本研究利用哺乳动物表达系统成功制备了重组PPP6C蛋白，并证实其与调控亚基PPP6R的结合能力。通过体外磷酸酶活性实验，发现重组PPP6C对特定磷酸化底物(如γ-H2AX)具有去磷酸化功能，提示其在DNA损伤修复中的作用。
2. **文献名称**：*"Structural Basis of PPP6C Substrate Specificity Revealed by Cryo-EM Analysis"*
**作者**：Wang et al.
**摘要**：作者通过重组PPP6C蛋白的冷冻电镜结构解析，揭示了其与底物结合的活性位点特征，并证明其催化活性依赖于Mn²⁺离子。研究为开发靶向PPP6C的小分子抑制剂提供了结构基础。
3. **文献名称**：*"PPP6C Recombinant Protein Rescues Mitotic Defects in PPP6C-Deficient Cancer Cells"*
**作者**：Tanaka et al.
**摘要**：在PPP6C敲除的癌细胞中，外源性添加重组PPP6C蛋白可恢复细胞周期进程并纠正染色体分离异常，证明PPP6C在维持有丝分裂保真性中的关键作用。
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**备注**：以上文献为示例，实际研究中建议通过PubMed或Web of Science等平台以关键词“PPP6C recombinant”“PPP6C purification”等检索最新文献。
**Background of PPP6C Recombinant Protein**
PPP6C, a catalytic subunit of protein phosphatase 6 (PP6), belongs to the serine/threonine phosphatase family, which plays a critical role in cellular signaling and homeostasis. PP6 is part of the evolutionarily conserved PPP (phosphoprotein phosphatase) family, sharing structural and functional similarities with PP1. PP2A, and PP5. The PPP6C subunit is essential for the enzyme’s activity, forming a trimeric holoenzyme with regulatory subunits such as PPP6R1-3. It is involved in diverse processes, including DNA damage repair, cell cycle regulation, immune response, and apoptosis.
Recombinant PPP6C protein is engineered through molecular cloning, where the *PPP6C* gene is expressed in heterologous systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein. This allows researchers to study its biochemical properties, substrate interactions, and regulatory mechanisms in vitro. PPP6C’s enzymatic activity is tightly regulated by binding partners and post-translational modifications, impacting pathways like NF-κB and Wnt signaling. Dysregulation of PPP6C has been linked to cancers, neurodegenerative disorders, and autoimmune diseases, making it a potential therapeutic target.
The recombinant protein is vital for structural studies (e.g., crystallography), inhibitor screening, and functional assays to dissect its role in health and disease. Its production ensures consistent quality and avoids contamination from native cellular proteins, facilitating high-precision research. Understanding PPP6C’s mechanisms through recombinant tools may unveil novel strategies for modulating phosphatase-related pathologies.
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