| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CABP1 |
| Uniprot No | P29377 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-79aa |
| 氨基酸序列 | MSTKKSPEELKRIFEKYAAKEGDPDQLSKDELKLLIQAEFPSLLKGPNTLDDLFQELDKNGDGEVSFEEFQVLVKKISQ |
| 预测分子量 | 9,0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CABP1重组蛋白的模拟参考文献示例(仅供参考，实际文献请通过学术数据库查询)：
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1. **标题**: *"Expression and Purification of Recombinant CABP1 in E. coli for Functional Studies"*
**作者**: Zhang L., et al. (Institute of Biochemistry, Chinese Academy of Sciences)
**摘要**: 研究利用大肠杆菌系统高效表达CABP1重组蛋白，通过优化诱导条件和镍柱亲和层析纯化，获得高纯度蛋白，并验证其钙离子结合活性。
2. **标题**: *"Structural Insights into Calcium Binding by CABP1 via X-ray Crystallography"*
**作者**: Müller R., et al. (Max Planck Institute for Molecular Physiology)
**摘要**: 解析CABP1重组蛋白的晶体结构，揭示其EF-hand结构域中钙离子的配位模式，探讨构象变化对下游信号调控的影响。
3. **标题**: *"CABP1 Modulates Neuronal Calcium Signaling through Interaction with Voltage-Gated Channels"*
**作者**: Chen H., Wang Y. (Stanford University School of Medicine)
**摘要**: 通过体外重组CABP1蛋白与神经元电压门控钙通道的共沉淀实验，证明其通过竞争性结合调控细胞内钙稳态及突触可塑性。
4. **标题**: *"Development of a CABP1-Based Fluorescent Biosensor for Real-Time Calcium Detection"*
**作者**: Tanaka K., et al. (RIKEN Center for Biosystems Dynamics Research)
**摘要**: 将重组CABP1蛋白与荧光标记融合，构建新型生物传感器，实现活细胞中钙动力学的实时监测，并验证其灵敏度和特异性。
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**备注**：以上内容为模拟生成，实际文献需通过PubMed、Google Scholar等平台以“CABP1 recombinant protein”“CABP1 expression”等关键词检索。
CABP1 (calcium-binding protein 1) is a member of the calcium-binding protein family that plays a role in modulating intracellular calcium signaling, a critical regulator of diverse cellular processes such as neurotransmission, gene expression, and enzyme activity. Structurally, it contains EF-hand motifs, helix-loop-helix domains that bind Ca²⁺ ions with high affinity, enabling it to act as a calcium sensor. Unlike the broader expressed calmodulin, CABP1 exhibits tissue-specific expression, predominantly in the brain and retina, suggesting specialized roles in neuronal and visual functions.
Recombinant CABP1 protein is engineered through heterologous expression systems (e.g., E. coli, mammalian cells) to produce purified, functional protein for research. Its recombinant form retains calcium-binding capacity and interaction partners, making it valuable for studying calcium-dependent signaling pathways. Researchers utilize it to explore CABP1’s regulatory effects on voltage-gated calcium channels, synaptic plasticity, and retinal phototransduction. Notably, CABP1 may influence presynaptic neurotransmitter release and postsynaptic receptor trafficking, linking it to neurological disorders like epilepsy or neurodegeneration.
Additionally, recombinant CABP1 aids in structural studies (e.g., X-ray crystallography) to resolve its Ca²⁺-bound vs. apo-state conformations, providing insights into activation mechanisms. It’s also used to develop antibodies, screen drug candidates targeting calcium signaling, or model diseases associated with calcium dysregulation. Emerging evidence implicates CABP1 in neurodevelopmental and neurodegenerative conditions, positioning it as a potential biomarker or therapeutic target. Overall, recombinant CABP1 serves as a pivotal tool for dissecting calcium-mediated cellular dynamics and their pathological disruptions.
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