| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CABP2 |
| Uniprot No | P56545 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-445aa |
| 氨基酸序列 | MALVDKHKVKRQRLDRICEGIRPQIMNGPLHPRPLVALLDGRDCTVEMPI LKDLATVAFCDAQSTQEIHEKVLNEAVGAMMYHTITLTREDLEKFKALRV IVRIGSGYDNVDIKAAGELGIAVCNIPSAAVEETADSTICHILNLYRRNT WLYQALREGTRVQSVEQIREVASGAARIRGETLGLIGFGRTGQAVAVRAK AFGFSVIFYDPYLQDGIERSLGVQRVYTLQDLLYQSDCVSLHCNLNEHNH HLINDFTIKQMRQGAFLVNAARGGLVDEKALAQALKEGRIRGAALDVHES EPFSFAQGPLKDAPNLICTPHTAWYSEQASLEMREAAATEIRRAITGRIP ESLRNCVNKEFFVTSAPWSVIDQQAIHPELNGATYRYPPGIVGVAPGGLP AAMEGIIPGGIPVTHNLPTVAHPSQAPSPNQPTKHGDNREHPNEQ |
| 预测分子量 | 75 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CABP2重组蛋白的3篇代表性文献信息：
1. **文献名称**: "Cloning, expression, and characterization of CABP2 in mammalian cells"
**作者**: Smith A et al.
**摘要**: 研究报道了CABP2基因的克隆及其在哺乳动物细胞中的重组表达，通过体外实验验证了重组蛋白的钙离子结合能力，并分析了其在信号通路中的潜在功能。
2. **文献名称**: "Structural and functional analysis of CABP2 using recombinant protein purification"
**作者**: Lee J et al.
**摘要**: 该文献描述了大肠杆菌中重组CABP2蛋白的高效表达与纯化方法，结合X射线晶体学解析了其三维结构，揭示了钙离子结合位点的关键氨基酸残基。
3. **文献名称**: "CABP2 mutations impair synaptic transmission via altered calcium sensing"
**作者**: Wang Y et al.
**摘要**: 通过重组CABP2蛋白的体外功能实验，发现与耳聋相关的CABP2突变体降低了钙离子亲和力，导致神经元突触传递异常，阐明了其病理机制。
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**备注**：以上文献为示例性内容，实际研究中建议通过PubMed或Web of Science以“CABP2 recombinant protein”为关键词检索最新论文。若需具体文献，可提供更详细的研究方向(如疾病机制、结构解析等)。
**Background of CABP2 Recombinant Protein**
CABP2 (Calcium-Binding Protein 2) is a member of the calcium-binding protein family, which plays critical roles in calcium-mediated signaling pathways. It is highly expressed in sensory systems, particularly the inner ear and retina, where calcium signaling is essential for neurotransmission and sensory processing. CABP2 interacts with voltage-gated calcium channels (VGCCs) and other calcium-sensitive proteins, modulating intracellular calcium homeostasis and synaptic vesicle release. Dysregulation of CABP2 has been linked to hearing impairments, as mutations in the *CABP2* gene are associated with autosomal recessive nonsyndromic hearing loss in humans.
Recombinant CABP2 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for structural and functional studies. This allows researchers to investigate its calcium-binding properties, interaction partners, and mechanistic role in sensory transduction. Structural analyses, such as X-ray crystallography or NMR, have revealed conserved EF-hand motifs critical for calcium ion coordination. Additionally, recombinant CABP2 is used in *in vitro* assays to study its impact on calcium channel kinetics and synaptic plasticity models.
Research on CABP2 also extends to potential therapeutic applications, particularly in understanding and treating hearing disorders. By elucidating how CABP2 mutations disrupt calcium signaling in hair cells, studies aim to develop targeted interventions. Overall, CABP2 recombinant protein serves as a vital tool for decoding calcium-dependent processes in sensory biology and disease pathology.
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