| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRTC1 |
| Uniprot No | Q6UUV9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-634aa |
| 氨基酸序列 | MATSNNPRKF SEKIALHNQK QAEETAAFEE VMKDLSLTRA ARLQLQKSQY LQLGPSRGQY YGGSLPNVNQ IGSGTMDLPF QTPFQSSGLD TSRTTRHHGL VDRVYRERGR LGSPHRRPLS VDKHGRQADS CPYGTMYLSP PADTSWRRTN SDSALHQSTM TPTQPESFSS GSQDVHQKRV LLLTVPGMEE TTSEADKNLS KQAWDTKKTG SRPKSCEVPG INIFPSADQE NTTALIPATH NTGGSLPDLT NIHFPSPLPT PLDPEEPTFP ALSSSSSTGN LAANLTHLGI GGAGQGMSTP GSSPQHRPAG VSPLSLSTEA RRQQASPTLS PLSPITQAVA MDALSLEQQL PYAFFTQAGS QQPPPQPQPP PPPPPASQQP PPPPPPQAPV RLPPGGPLLP SASLTRGPQP PPLAVTVPSS LPQSPPENPG QPSMGIDIAS APALQQYRTS AGSPANQSPT SPVSNQGFSP GSSPQHTSTL GSVFGDAYYE QQMAARQANA LSHQLEQFNM MENAISSSSL YSPGSTLNYS QAAMMGLTGS HGSLPDSQQL GYASHSGIPN IILTVTGESP PSLSKELTSS LAGVGDVSFD SDSQFPLDEL KIDPLTLDGL HMLNDPDMVL ADPATEDTFR MDRL |
| 预测分子量 | 67,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CRTC1重组蛋白的3篇参考文献示例(注：文献为虚拟示例，实际引用请核实真实来源)：
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1. **文献名称**：*CRTC1 Recombinant Protein Modulates Hepatic Glucose Production via CREB Interaction*
**作者**：Zhang L, et al.
**摘要**：研究通过重组CRTC1蛋白表达，揭示了其在肝脏细胞中通过结合CREB调控糖异生基因的分子机制，为代谢性疾病治疗提供新靶点。
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2. **文献名称**：*Structural Insights into CRTC1 Recombinant Protein Activation by Calcium Signaling*
**作者**：Murakami T, et al.
**摘要**：利用重组CRTC1蛋白解析其钙离子依赖的构象变化，阐明其在神经元突触可塑性中的调控作用，揭示与认知功能相关的结构基础。
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3. **文献名称**：*Recombinant CRTC1 Enhances Tumor Suppression in Colorectal Cancer Models*
**作者**：Chen H, et al.
**摘要**：通过体外重组CRTC1蛋白递送实验，证明其通过抑制Wnt/β-catenin通路抑制结直肠癌细胞增殖，提示潜在抗癌应用价值。
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(如需真实文献，建议在PubMed或Google Scholar中检索关键词“CRTC1 recombinant protein”或结合具体研究领域筛选。)
**Background of CRTC1 Recombinant Protein**
CRTC1 (CREB-regulated transcription coactivator 1) is a key regulatory protein involved in the cAMP-responsive element-binding protein (CREB) signaling pathway. As a member of the CRTC family, it functions as a coactivator by binding to CREB, enhancing its transcriptional activity in response to extracellular signals such as hormonal cues, cellular stress, or neuronal activity. CRTC1 is particularly notable for its role in energy homeostasis, synaptic plasticity, and memory formation, with significant expression in brain regions like the hippocampus and hypothalamus.
Dysregulation of CRTC1 has been linked to various diseases, including metabolic disorders, neurodegenerative conditions (e.g., Alzheimer’s disease), and cancer. For instance, aberrant CRTC1 activity may disrupt glucose metabolism or promote tumorigenesis by modulating oncogenic gene networks. Its phosphorylation-dependent nucleocytoplasmic shuttling—regulated by kinases such as AMPK and LKB1—ensures tight control over its transcriptional functions, making it a critical node in stress-response pathways.
Recombinant CRTC1 protein is engineered for in vitro and in vivo studies to dissect its molecular interactions, post-translational modifications, and regulatory mechanisms. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), it retains functional domains required for CREB binding and transcriptional activation. Researchers utilize this tool to explore CRTC1’s role in disease models, screen for therapeutic agents, or analyze structure-function relationships. Its application also extends to elucidating how environmental or genetic perturbations alter CRTC1-driven gene programs, offering insights into targeted therapies for related pathologies.
Overall, CRTC1 recombinant protein serves as a vital resource for understanding CREB-mediated gene regulation and its implications in health and disease.
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