| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDT |
| Uniprot No | P30046 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-118aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMPFLELDTNLPANRVPAGLEKRLCAAAASI LGKPADRVNVTVRPGLAMALSGSTEPCAQLSISSIGVVGTAEDNRSHSAH FFEFLTKELALGQDRILIRFFPLESWQIGKIGTVMTFL |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DDT重组蛋白的3篇示例文献(注：以下内容为虚构示例，仅供参考格式)：
1. **文献名称**：*Expression and Purification of Recombinant DDT-Binding Protein for Environmental Monitoring*
**作者**：Chen H, et al.
**摘要**：研究通过大肠杆菌系统高效表达并纯化一种DDT结合重组蛋白，验证其与DDT的特异性结合能力，为环境污染物检测提供新型生物传感器材料。
2. **文献名称**：*Engineering a Recombinant DDT-Degrading Enzyme via Directed Evolution*
**作者**：Kumar S, Lee J.
**摘要**：利用定向进化技术改造天然脱卤酶，获得重组蛋白变体，显著提升对DDT的催化降解效率，为土壤修复提供潜在工具。
3. **文献名称**：*Structural Insights into DDT Resistance Mediated by Recombinant CYP6G1 Protein in Drosophila*
**作者**：Martinez P, et al.
**摘要**：解析果蝇CYP6G1重组蛋白的晶体结构，揭示其代谢DDT的分子机制，阐明昆虫抗药性产生的结构基础。
4. **文献名称**：*Recombinant Antibody-Based Detection of DDT Residues in Agricultural Products*
**作者**：Zhang Y, et al.
**摘要**：开发基于重组单链抗体的免疫检测方法，实现对农产品中痕量DDT残留的高灵敏度、低成本筛查。
(注：实际文献需通过PubMed、Google Scholar等平台检索确认。)
**Background of DDT Recombinant Protein**
DDT (D-dopachrome tautomerase), also known as MIF-2 (macrophage migration inhibitory factor-2), is a multifunctional protein belonging to the MIF cytokine family. It shares structural and functional similarities with MIF, including a conserved catalytic tautomerase site, though its biological roles are distinct. Discovered in the 1990s, DDT is encoded by the *DDT* gene and is evolutionarily conserved across species, highlighting its physiological significance.
Structurally, DDT forms homotrimers and interacts with cell surface receptors such as CD74. albeit with different binding affinities compared to MIF. It exhibits tautomerase activity, converting D-dopachrome into intermediates like 5.6-dihydroxyindole, though the physiological relevance of this activity remains under investigation. DDT is implicated in immune regulation, inflammation, and cellular stress responses. It modulates pathways like MAPK and PI3K/Akt, influencing cell proliferation, apoptosis, and cytokine production.
Research links DDT to diseases including cancer, autoimmune disorders, and neurodegenerative conditions. For instance, it is overexpressed in certain tumors, promoting angiogenesis and metastasis, while in Alzheimer’s disease, it may contribute to neuroinflammation.
Recombinant DDT protein is produced using prokaryotic (e.g., *E. coli*) or eukaryotic expression systems to ensure proper folding and post-translational modifications. Purification techniques like affinity chromatography (e.g., His-tag) yield high-purity protein for research. It serves as a tool to study DDT’s mechanisms, receptor interactions, and therapeutic potential. Inhibitors targeting DDT are being explored for conditions like sepsis or cancer.
Overall, DDT recombinant protein is vital for deciphering its biological roles and developing targeted therapies, bridging gaps in understanding immune and disease pathways.
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