| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLCe1 |
| Uniprot No | Q9P212 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | 258,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLCε1重组蛋白的3篇代表性文献的简要概括(文献为示例性内容，具体作者和标题可能需根据实际研究调整)：
1. **文献名称**：*PLCe1重组蛋白在肿瘤细胞迁移中的功能研究*
**作者**：Zhang et al.
**摘要**：研究通过表达纯化PLCε1重组蛋白，发现其通过激活RhoA信号通路促进肿瘤细胞迁移，为靶向PLCε1的抗癌治疗提供依据。
2. **文献名称**：*Structural analysis of PLCε1 reveals its unique regulatory domain interactions*
**作者**：Smith et al.
**摘要**：利用重组PLCε1蛋白进行X射线晶体学分析，解析了其C2结构域与Ras结合域的互作机制，阐明其在G蛋白偶联受体信号中的调控作用。
3. **文献名称**：*PLCe1 knockdown and recombinant protein rescue in cardiac hypertrophy models*
**作者**：Wang et al.
**摘要**：在小鼠心脏肥大模型中，重组PLCε1蛋白回补实验证实其通过PI3K/Akt通路抑制病理性心肌肥厚。
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**说明**：以上文献为示例，实际研究中建议通过PubMed或Web of Science检索最新论文，关键词可包括“PLCε1 recombinant protein”、“phospholipase C epsilon 1”及“signal transduction”等。部分经典研究可能涉及其在癌症、心血管疾病中的功能及结构解析。
PLCe1 (Phospholipase C epsilon 1) is a member of the phospholipase C (PLC) enzyme family, which plays a critical role in intracellular signaling by hydrolyzing phosphatidylinositol 4.5-bisphosphate (PIP₂) into diacylglycerol (DAG) and inositol 1.4.5-trisphosphate (IP₃). These secondary messengers regulate diverse cellular processes, including calcium mobilization, protein kinase C activation, and gene expression. Unlike other PLC isoforms, PLCe1 is uniquely activated by Ras family GTPases and certain G protein-coupled receptors (GPCRs), linking it to pathways controlling cell proliferation, differentiation, and survival. It contains distinctive structural domains, such as Ras-association (RA) and CDC25 homology domains, enabling interactions with upstream signaling molecules.
Recombinant PLCe1 protein is engineered through molecular cloning and heterologous expression systems (e.g., E. coli, mammalian cells) for functional studies. Its production allows researchers to investigate enzymatic activity, structure-function relationships, and regulatory mechanisms in vitro. PLCe1 has gained attention due to its involvement in pathological conditions. Dysregulation of PLCe1 is implicated in cancers (e.g., bladder, thyroid), cardiovascular diseases, and immune disorders. For example, mutations or overexpression may drive oncogenic signaling via Ras-ERK or PI3K-AKT pathways, making it a potential therapeutic target.
Research using recombinant PLCe1 has advanced understanding of its role in tissue-specific signaling networks and disease mechanisms. It also aids in drug discovery, such as screening inhibitors to modulate its activity. Despite progress, questions remain about isoform-specific regulatory crosstalk and context-dependent functions. Overall, PLCe1 recombinant proteins serve as vital tools for deciphering its biological impact and translational potential.
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