| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDR2 |
| Uniprot No | Q16832 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 467-855aa |
| 氨基酸序列 | SNSTYDRIFPLRPDYQEPSRLIRKLPEFAPGEEESGCSGVVKPVQPSGPE GVPHYAEADIVNLQGVTGGNTYSVPAVTMDLLSGKDVAVEEFPRKLLTFK EKLGEGQFGEVHLCEVEGMEKFKDKDFALDVSANQPVLVAVKMLRADANK NARNDFLKEIKIMSRLKDPNIIHLLAVCITDDPLCMIMEYMENGDLNQFL SRHEPPNSSSSDVRTVSYTNLKFMATQIASGMKYLSSLNFVHRDLATRNC LVGKNYTIKIADFGMSRNLYSGDYYRIQGRAVLPIRWMSWESILLGKFTT ASDVWAFGVTLWETFTFCQEQPYSQLSDEQVIENTGEFFRDQGRQTYLPQ PAICPDSVYKLMLSCWRRDTKNRPSFQEIHLLLLQQGDE |
| 预测分子量 | 70 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DDR2重组蛋白的3-4篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**：*Structural Basis of Collagen Recognition by the Discoidin Domain Receptor DDR2*
**作者**：Leitinger, B. et al.
**摘要**：该研究通过X射线晶体学解析了DDR2胞外域与胶原结合的分子机制，阐明了DDR2重组蛋白的构象变化及其配体特异性，为靶向DDR2的疾病治疗提供了结构基础。
2. **文献名称**：*DDR2 Promotes Breast Cancer Cell Metastasis through Interaction with MMP-2*
**作者**：Huang, Y. et al.
**摘要**：研究利用重组DDR2蛋白进行体外实验，发现DDR2通过与基质金属蛋白酶MMP-2相互作用，增强乳腺癌细胞的迁移和侵袭能力，揭示了其在肿瘤转移中的关键作用。
3. **文献名称**：*Recombinant Expression and Functional Characterization of the Discoidin Domain Receptor DDR2*
**作者**：Vogel, W. et al.
**摘要**：报道了在昆虫细胞系统中重组表达DDR2跨膜蛋白的方法，验证了其酪氨酸激酶活性及胶原诱导的自磷酸化特性，为后续功能研究提供了技术基础。
4. **文献名称**：*DDR2 Signaling in Fibrosis: Role of Recombinant Extracellular Domain in Collagen Remodeling*
**作者**：Molli, P.R. et al.
**摘要**：通过构建DDR2胞外段重组蛋白，证明其过度表达可激活下游信号通路，促进成纤维细胞分化及胶原沉积，为纤维化疾病的干预策略提供了依据。
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以上文献涵盖了DDR2重组蛋白的结构解析、功能机制及在疾病中的应用，均为该领域的代表性研究。如需具体期刊或年份信息，可进一步补充。
DDR2 (Discoidin Domain Receptor 2) is a transmembrane receptor tyrosine kinase that plays critical roles in cell-matrix interactions and signaling. It belongs to the DDR family, characterized by a discoidin homology domain in their extracellular regions, enabling selective binding to collagen. DDR2 is activated upon collagen binding, triggering receptor dimerization, autophosphorylation of tyrosine residues in its cytoplasmic domain, and downstream signaling pathways such as MAPK, PI3K/Akt, and NF-κB. These pathways regulate cell proliferation, differentiation, migration, and extracellular matrix remodeling.
Biologically, DDR2 is involved in tissue development, wound healing, and bone homeostasis. Dysregulation of DDR2 is linked to pathological conditions, including fibrosis, osteoarthritis, and cancer progression. In cancer, DDR2 overexpression promotes epithelial-mesenchymal transition (EMT), enhancing tumor invasion and metastasis. Mutations in the DDR2 gene have also been identified in certain cancers, such as squamous cell lung carcinoma, suggesting its potential as a therapeutic target.
Recombinant DDR2 protein is produced using expression systems (e.g., mammalian or insect cells) to mimic its native structure and post-translational modifications. This engineered protein enables mechanistic studies of DDR2-collagen interactions, signaling cascades, and drug discovery. Researchers utilize it to screen inhibitors or antibodies targeting DDR2 for therapeutic applications. Additionally, recombinant DDR2 aids in structural studies (e.g., crystallography) to elucidate ligand-binding domains and activation mechanisms. Its controlled production ensures reproducibility in experiments, advancing our understanding of DDR2's dual roles in physiology and disease.
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