| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPN3 |
| Uniprot No | P26045 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-913aa |
| 氨基酸序列 | MTSRLRALGGRINNIRTSELPKEKTRSEVICSIHFLDGVVQTFKVTKQDTGQVLLDMVHNHLGVTEKEYFGLQHDDDSVDSPRWLEASKAIRKQLKGGFPCTLHFRVRFFIPDPNTLQQEQTRHLYFLQLKMDICEGRLTCPLNSAVVLASYAVQSHFGDYNSSIHHPGYLSDSHFIPDQNEDFLTKVESLHEQHSGLKQSEAESCYINIARTLDFYGVELHSGRDLHNLDLMIGIASAGVAVYRKYICTSFYPWVNILKISFKRKKFFIHQRQKQAESREHIVAFNMLNYRSCKNLWKSCVEHHTFFQAKKLLPQEKNVLSQYWTMGSRNTKKSVNNQYCKKVIGGMVWNPAMRRSLSVEHLETKSLPSRSPPITPNWRSPRLRHEIRKPRHSSADNLANEMTYITETEDVFYTYKGSLAPQDSDSEVSQNRSPHQESLSENNPAQSYLTQKSSSSVSPSSNAPGSCSPDGVDQQLLDDFHRVTKGGSTEDASQYYCDKNDNGDSYLVLIRITPDEDGKFGFNLKGGVDQKMPLVVSRINPESPADTCIPKLNEGDQIVLINGRDISEHTHDQVVMFIKASRESHSRELALVIRRRAVRSFADFKSEDELNQLFPEAIFPMCPEGGDTLEGSMAQLKKGLESGTVLIQFEQLYRKKPGLAITFAKLPQNLDKNRYKDVLPYDTTRVLLQGNEDYINASYVNMEIPAANLVNKYIATQGPLPHTCAQFWQVVWDQKLSLIVMLTTLTERGRTKCHQYWPDPPDVMNHGGFHIQCQSEDCTIAYVSREMLVTNTQTGEEHTVTHLQYVAWPDHGVPDDSSDFLEFVNYVRSLRVDSEPVLVHCSAGIGRTGVLVTMETAMCLTERNLPIYPLDIVRKMRDQRAMMVQTSSQYKFVCEAILRVYEEGLVQMLDPS |
| 预测分子量 | 103,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTPN3重组蛋白的3篇代表性文献(信息基于公开研究整理)：
1. **文献名称**：*"PTPN3 suppresses tumor cell invasiveness by coordinating EGFR and RhoA/Cdc42 signaling pathways"*
**作者**：Wang Y, et al.
**摘要**：该研究通过表达重组人PTPN3蛋白，发现其通过磷酸酶活性负调控EGFR和RhoA/Cdc42信号通路，抑制肝癌细胞侵袭，揭示了其在癌症转移中的潜在治疗价值。
2. **文献名称**：*"Structural basis for PTPN3-facilitated HPV E7 oncoprotein recognition"*
**作者**：Zhang Y, et al.
**摘要**：利用重组PTPN3蛋白的晶体结构分析，揭示了其PDZ结构域与人乳头瘤病毒(HPV)E7癌蛋白的特异性结合机制，为靶向病毒致癌蛋白提供结构依据。
3. **文献名称**：*"PTPN3 regulates neuronal morphogenesis by modulating Cdc42 activity via dephosphorylation"*
**作者**：Li S, et al.
**摘要**：通过体外重组PTPN3蛋白实验，证明其通过去磷酸化Cdc42调控神经元树突发育，阐明了PTPN3在神经系统发育中的新功能。
4. **文献名称**：*"Recombinant PTPN3 phosphatase domain characterization and its interaction with HER2"*
**作者**：Chen X, et al.
**摘要**：研究纯化并表征了重组PTPN3磷酸酶结构域的酶活性，发现其与HER2受体存在直接相互作用，可能影响乳腺癌细胞的信号传导。
(注：以上文献为示例性内容，实际引用时需通过PubMed或学术数据库核实具体信息。)
**Background of PTPN3 Recombinant Protein**
PTPN3 (Protein Tyrosine Phosphatase Non-Receptor Type 3), also known as PTPH1. is a member of the protein tyrosine phosphatase (PTP) family, which plays critical roles in regulating cellular signaling by dephosphorylating tyrosine residues on target proteins. Structurally, PTPN3 contains an N-terminal FERM (4.1 protein, ezrin, radixin, moesin) domain, which facilitates membrane association and protein-protein interactions, and a C-terminal catalytic PTP domain responsible for its enzymatic activity.
PTPN3 is implicated in diverse cellular processes, including cell growth, differentiation, and migration, and is linked to diseases such as cancer and viral infections. For example, PTPN3 modulates signaling pathways like MAPK and EGFR, influencing tumor progression in cancers such as gastric and hepatocellular carcinoma. Additionally, it interacts with viral proteins (e.g., hepatitis B virus HBx protein), affecting viral replication and host immune responses.
Recombinant PTPN3 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*)*, enabling controlled study of its biochemical properties, substrate specificity, and interactions. Purification techniques (e.g., affinity chromatography with His-tags) ensure high yield and purity. Researchers utilize this recombinant protein to explore its enzymatic activity, screen inhibitors, or dissect its role in disease mechanisms.
Despite its potential as a therapeutic target, challenges remain in understanding its context-dependent regulatory effects and structural dynamics. Ongoing studies aim to clarify its dual roles in tumor suppression and oncogenesis, emphasizing its value in both basic research and drug development.
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