| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APBB2 |
| Uniprot No | Q92870 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-758aa |
| 氨基酸序列 | MSEVLPADSG VDTLAVFMAS SGTTDVTNRN SPATPPNTLN LRSSHNELLN AEIKHTETKN STPPKCRKKY ALTNIQAAMG LSDPAAQPLL GNGSANIKLV KNGENQLRKA AEQGQQDPNK NLSPTAVINI TSEKLEGKEP HPQDSSSCEI LPSQPRRTKS FLNYYADLET SARELEQNRG NHHGTAEEKS QPVQGQASTI IGNGDLLLQK PNRPQSSPED GQVATVSSSP ETKKDHPKTG AKTDCALHRI QNLAPSDEES SWTTLSQDSA SPSSPDETDI WSDHSFQTDP DLPPGWKRVS DIAGTYYWHI PTGTTQWERP VSIPADLQGS RKGSLSSVTP SPTPENEKQP WSDFAVLNGG KINSDIWKDL HAATVNPDPS LKEFEGATLR YASLKLRNAP HPDDDDSCSI NSDPEAKCFA VRSLGWVEMA EEDLAPGKSS VAVNNCIRQL SYCKNDIRDT VGIWGEGKDM YLILENDMLS LVDPMDRSVL HSQPIVSIRV WGVGRDNGRD FAYVARDKDT RILKCHVFRC DTPAKAIATS LHEICSKIMA ERKNAKALAC SSLQERANVN LDVPLQVDFP TPKTELVQKF HVQYLGMLPV DKPVGMDILN SAIENLMTSS NKEDWLSVNM NVADATVTVI SEKNEEEVLV ECRVRFLSFM GVGKDVHTFA FIMDTGNQRF ECHVFWCEPN AGNVSEAVQA ACMLRYQKCL VARPPSQKVR PPPPPADSVT RRVTTNVKRG VLSLIDTLKQ KRPVTEMP |
| 预测分子量 | 83,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于APBB2重组蛋白的3篇代表性文献的简要信息：
1. **文献名称**: *APBB2 modulates Aβ production by interacting with amyloid precursor protein*
**作者**: Zhou Z, et al.
**摘要**: 该研究通过体外重组APBB2蛋白实验，发现APBB2与淀粉样前体蛋白(APP)直接结合，并通过调节γ-分泌酶活性影响β淀粉样蛋白(Aβ)的生成，提示其在阿尔茨海默病病理中的潜在作用。
2. **文献名称**: *Structural insights into the interaction between APBB2 and the intracellular domain of APP*
**作者**: Kim S, et al.
**摘要**: 利用重组APBB2蛋白和X射线晶体学技术，揭示了APBB2的磷酸酪氨酸结合结构域(PTB)与APP胞内结构域的结合模式，阐明了二者相互作用的关键氨基酸位点。
3. **文献名称**: *APBB2 regulates neuronal development via endocytic trafficking*
**作者**: Wang L, et al.
**摘要**: 通过表达重组APBB2蛋白并分析其在神经元中的功能，发现APBB2通过调控网格蛋白介导的内吞作用影响神经突触形成和轴突导向，表明其参与神经发育的分子机制。
(注：以上文献信息为模拟概括，实际文献需通过PubMed或专业数据库检索确认。)
APBB2 (Amyloid Beta Precursor Protein Binding Family B Member 2), also known as Fe65L or X11L, is a multidomain adaptor protein involved in diverse cellular processes, including signal transduction, protein trafficking, and transcriptional regulation. It belongs to the Fe65 protein family, characterized by conserved phosphotyrosine-binding (PTB) domains that mediate interactions with the intracellular domain of amyloid precursor protein (APP), a key player in Alzheimer’s disease pathogenesis. APBB2 is implicated in modulating APP processing, influencing the production of amyloid-beta (Aβ) peptides, which aggregate into toxic plaques in Alzheimer’s brains. Beyond its role in neurodegeneration, APBB2 participates in cytoskeletal organization, receptor endocytosis, and cell adhesion through interactions with proteins like Mena, LRP1. and transcription factors.
Recombinant APBB2 proteins are engineered to study its biochemical functions, structural features, and interactions in vitro. These proteins are typically produced in heterologous expression systems (e.g., E. coli or mammalian cells) and purified using affinity tags. Recombinant APBB2 retains functional domains, such as the PTB domain and WW motif, enabling researchers to dissect its role in APP processing pathways or its interplay with other signaling molecules like Tip60 or PI3K. Studies using recombinant APBB2 have clarified its dual role in promoting Aβ generation via APP stabilization while also regulating gene expression through nuclear translocation. Additionally, recombinant APBB2 serves as a tool for drug discovery, particularly in screening compounds targeting Aβ-related pathways. Its structural analysis (e.g., via X-ray crystallography) further aids in understanding molecular mechanisms underlying Alzheimer’s and other APBB2-associated disorders, such as cancer and metabolic syndromes.
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