| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COX6c |
| Uniprot No | P09669 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-75aa |
| 氨基酸序列 | MAPEVLPKPRMRGLLARRLRNHMAVAFVLSLGVAALYKFRVADQRKKAYADFYRNYDVMKDFEEMRKAGIFQSVK |
| 预测分子量 | 8,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COX6c重组蛋白的3篇代表性文献(注：以下为模拟示例，实际文献需通过数据库验证)：
1. **《Functional characterization of recombinant COX6c in mitochondrial electron transport chain assembly》**
- **作者**: Zhang L, et al.
- **摘要**: 研究通过在大肠杆菌中重组表达人源COX6c蛋白，分析其在线粒体复合物IV组装中的作用，证实其与COX5a的互作对酶活性调控至关重要。
2. **《COX6c overexpression promotes tumor growth via modulating cellular metabolism in lung adenocarcinoma》**
- **作者**: Kim S, Park JH.
- **摘要**: 利用哺乳动物细胞系表达重组COX6c，发现其过表达通过增强线粒体呼吸链功能促进肺癌细胞增殖，提示其作为癌症治疗靶点的潜力。
3. **《Structural insights into COX6c and its role in hypoxia adaptation》**
- **作者**: Rossi M, et al.
- **摘要**: 通过重组COX6c蛋白的晶体结构解析，揭示其低氧条件下稳定复合物IV构象的分子机制，为缺氧相关疾病研究提供依据。
建议通过PubMed或Web of Science检索最新文献，关键词包括“COX6c recombinant expression”或“COX6c protein function”。
**Background of COX6c Recombinant Protein**
Cytochrome c oxidase subunit 6C (COX6c), a nuclear-encoded subunit of mitochondrial Complex IV, plays a critical role in the electron transport chain (ETC) by facilitating the transfer of electrons from cytochrome c to molecular oxygen, coupled with proton pumping to support ATP synthesis. As part of the heme-copper oxidase superfamily, COX6c contributes to the structural stability and functional regulation of Complex IV, which is essential for cellular energy metabolism. Unlike some subunits of Complex IV, COX6c is not directly involved in catalytic activity but is thought to assist in maintaining the assembly or conformational dynamics of the enzyme.
The COX6c gene is located on human chromosome 8q22-q23. and its expression is tightly regulated in a tissue-specific manner, with high abundance in metabolically active tissues like the heart and brain. Dysregulation of COX6c has been linked to mitochondrial disorders, neurodegenerative diseases, and cancer. For instance, reduced COX6c levels impair mitochondrial respiration, potentially contributing to pathologies such as Leigh syndrome or Parkinson’s disease. In cancer, altered COX6c expression may influence tumor progression by modulating metabolic reprogramming.
Recombinant COX6c protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to study its biochemical properties, interactions, and disease associations. Purified via affinity tags (e.g., His-tag), the recombinant protein enables functional analyses, antibody production, and screening for therapeutic agents targeting mitochondrial dysfunction. Its application extends to deciphering molecular mechanisms of Complex IV deficiencies and exploring COX6c’s role in cellular adaptation to oxidative stress. Overall, COX6c recombinant protein serves as a vital tool for advancing mitochondrial research and therapeutic innovation.
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