| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCXR |
| Uniprot No | Q7Z4W1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-244aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMELFLAGRRVLVTGAGKGIGRGTVQALHAT GARVVAVSRTQADLDSLVRECPGIEPVCVDLGDWEATERALGSVGPVDLL VNNAAVALLQPFLEVTKEAFDRSFEVNLRAVIQVSQIVARGLIARGVPGA IVNVSSQCSQRAVTNHSVYCSTKGALDMLTKVMALELGPHKIRVNAVNPT VVMTSMGQATWSDPHKAKTMLNRIPLGKFAEVEHVVNAILFLLSDRSGMT TGSTLPVEGGFWAC |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DCXR(Dihydroxyacetone phosphate reductase)重组蛋白的模拟参考文献示例(注：以下为假设性内容，实际文献需通过学术数据库查询)：
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1. **文献名称**：*Recombinant Human DCXR: Expression, Purification, and Enzymatic Characterization*
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人源DCXR蛋白，分析了其还原酶活性，证实其对二羟基丙酮磷酸(DHAP)的催化效率，为后续代谢通路研究奠定基础。
2. **文献名称**：*DCXR Recombinant Protein Attenuates Oxidative Stress in Diabetic Nephropathy Models*
**作者**：Chen L, et al.
**摘要**：通过体外实验发现，重组DCXR蛋白可减少高糖诱导的肾细胞氧化损伤，提示其在糖尿病肾病中的潜在治疗作用。
3. **文献名称**：*Structural Insights into DCXR-mediated Fructose Metabolism via X-ray Crystallography*
**作者**：Yamamoto K, et al.
**摘要**：利用重组DCXR蛋白进行晶体结构解析，揭示了其底物结合域的关键氨基酸残基，阐明了其在果糖代谢中的分子机制。
4. **文献名称**：*DCXR as a Biomarker in Hepatocellular Carcinoma: Recombinant Protein-based Clinical Analysis*
**作者**：Wang X, et al.
**摘要**：通过重组DCXR蛋白制备抗体，发现肝癌患者血清中DCXR水平显著升高，提示其作为诊断标志物的可能性。
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建议通过PubMed或Google Scholar检索真实文献，关键词包括“DCXR recombinant protein”、“DCXR enzymatic activity”等。
DCXR (Dicarbonyl/L-Xylulose Reductase), also known as carbonyl reductase 3 or spermatogenic protein 34 (SP-34), is a multifunctional enzyme belonging to the short-chain dehydrogenase/reductase (SDR) superfamily. It catalyzes the NADPH-dependent reduction of dicarbonyl compounds (e.g., methylglyoxal) and L-xylulose, playing roles in cellular detoxification and glucose metabolism pathways. DCXR is unique among SDR enzymes for its dual substrate specificity and dimeric structure, distinguishing it from typical monomeric SDR members.
Recombinant DCXR protein is engineered through genetic cloning and expression systems (e.g., E. coli, yeast, or mammalian cells) to produce purified, functional enzyme for research and therapeutic applications. Its study gained momentum due to implications in diabetic complications, where elevated methylglyoxal levels contribute to cellular damage. DCXR's ability to detoxify reactive carbonyl species links it to aging-related pathologies and metabolic disorders, particularly through its interaction with advanced glycation end products (AGEs).
In reproductive biology, DCXR is highly expressed in sperm and oocytes, where it may influence sperm-egg recognition. This tissue-specific expression pattern has also drawn attention to its potential as a biomarker for certain cancers, including renal and liver malignancies, though its exact role in tumor progression remains debated.
Current research focuses on elucidating DCXR's structural dynamics, substrate-binding mechanisms, and pathophysiological significance. Recombinant forms enable high-throughput screening for inhibitors/activators, with therapeutic potential in diabetes management and AGE-related diseases. However, its paradoxical roles in both cytoprotection and potential tumorigenesis warrant further investigation to clarify context-dependent functions. Advanced production techniques now yield stable recombinant DCXR with preserved enzymatic activity, facilitating mechanistic studies and biomedical applications.
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