| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCTPP1 |
| Uniprot No | Q9H773 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-170aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMSVAGGEIRGDTGGEDTAAPGRFSFS PEPTLEDIRRLHAEFAAERDWEQFHQPRNLLLALVGEVGELAELFQWKTD GEPGPQGWSPRERAALQEELSDVLIYLVALAARCRVDLPLAVLSKMDINR RRYPAHLARSSSRKYTELPHGAISEDQAVGPADIPCDSTGQTST |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DCTPP1重组蛋白的3篇参考文献的简要概括：
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1. **文献名称**: *"Biochemical characterization of human DCTPP1 reveals a trimeric assembly and resistance to common pyrophosphatase inhibitors"*
**作者**: Smith J, et al.
**摘要**: 研究报道了人源DCTPP1重组蛋白在大肠杆菌中的表达与纯化，发现其以三聚体形式存在，并证明其对常见焦磷酸酶抑制剂不敏感，提示其催化机制可能与其他焦磷酸酶不同。
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2. **文献名称**: *"DCTPP1 prevents incorporation of 5-methyl-dCTP into DNA by hydrolyzing the modified nucleotide"*
**作者**: Tanaka H, et al.
**摘要**: 通过体外实验证实重组DCTPP1蛋白具有特异性水解5-甲基-dCTP的能力，表明其在维持基因组稳定性中起关键作用，可防止异常甲基化核苷酸整合到DNA中。
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3. **文献名称**: *"Structural insights into the substrate specificity of DCTPP1 through X-ray crystallography"*
**作者**: Li X, et al.
**摘要**: 解析了重组DCTPP1蛋白的晶体结构，揭示了其底物结合口袋的独特构象，解释了其对dCTP及其衍生物的特异性识别机制，为靶向药物设计提供了结构基础。
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**注**: 以上文献为示例，实际引用时需根据具体研究内容选择真实发表的论文。建议通过PubMed或Web of Science以“DCTPP1 recombinant protein”为关键词检索最新文献。
**Background of DCTPP1 Recombinant Protein**
DCTPP1 (DCTP pyrophosphatase 1), also known as RHOB or CDA03. is a nucleotidyl phosphatase enzyme encoded by the *DCTPP1* gene. It belongs to the HAM1 superfamily and plays a critical role in maintaining genomic stability by regulating nucleotide pool homeostasis. DCTPP1 specifically hydrolyzes dCTP and 5-methyl-dCTP into their corresponding monophosphates, preventing the misincorporation of non-canonical nucleotides during DNA replication and repair. This activity is vital for minimizing mutagenesis and ensuring DNA fidelity, particularly under conditions of cellular stress or metabolic imbalance.
Recombinant DCTPP1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, biologically active forms of the enzyme for research applications. Its recombinant form retains enzymatic specificity and is widely used to study nucleotide metabolism, DNA damage response pathways, and mechanisms of chemotherapeutic resistance. Studies have linked DCTPP1 dysfunction to pathologies such as cancer, where altered nucleotide metabolism can drive tumorigenesis, and neurodegenerative disorders, implicating its role in oxidative stress responses.
In drug discovery, recombinant DCTPP1 serves as a tool for screening inhibitors or modulators targeting nucleotide metabolism, with potential therapeutic applications in oncology. Structural and functional analyses of the recombinant protein have further elucidated its catalytic mechanism and interaction with substrates, aiding the design of targeted therapies. Overall, DCTPP1 recombinant protein is a key reagent for exploring cellular nucleotide dynamics and developing strategies to address diseases linked to genomic instability.
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