| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCTN6 |
| Uniprot No | O00399 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-190aa |
| 氨基酸序列 | MAEKTQKSVKIAPGAVVCVESEIRGDVTIGPRTVIHPKARIIAEAGPIVIGEGNLIEEQALIINAYPDNITPDTEDPEPKPMIIGTNNVFEVGCYSQAMKMGDNNVIESKAYVGRNVILTSGCIIGACCNLNTFEVIPENTVIYGADCLRRVQTERPQPQTLQLDFLMKILPNYHHLKKTMKGSSTPVKN |
| 预测分子量 | 47.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DCTN6重组蛋白的3篇参考文献示例(注：文献为虚构示例，实际研究需查阅真实数据库)：
1. **文献名称**：*Structural Insights into the Role of DCTN6 in Dynein-Dynactin Complex Assembly*
**作者**：Zhang, Y. et al.
**摘要**：本研究通过重组表达人源DCTN6蛋白，结合冷冻电镜技术解析了DCTN6与DCTN5的异源二聚体结构，揭示了其在动力蛋白-动力蛋白激活复合体(Dynein-Dynactin)中的关键结合界面，为理解细胞内运输机制提供结构基础。
2. **文献名称**：*Functional Characterization of Recombinant DCTN6 in Neuronal Vesicle Transport*
**作者**：Smith, J.R. & Lee, M.
**摘要**：利用重组DCTN6蛋白进行体外功能实验，发现其缺失会导致动力蛋白复合体与微管结合能力下降，并影响神经元内囊泡运输效率，表明DCTN6在神经退行性疾病中可能具有病理意义。
3. **文献名称**：*DCTN6 Recombinant Protein as a Novel Biomarker for Cancer Cell Motility*
**作者**：Chen, X. et al.
**摘要**：通过表达纯化DCTN6重组蛋白并分析其在多种癌细胞系中的表达水平，发现DCTN6高表达与细胞迁移能力正相关，提示其可能作为癌症转移的潜在诊断标记物。
(如需真实文献，建议在PubMed或Web of Science中以“DCTN6 recombinant protein”或“DCTN6 dynactin”为关键词检索。)
DCTN6 (Dynactin subunit 6) is a critical component of the dynactin complex, a multi-protein assembly essential for intracellular transport processes. As part of the dynactin family, it works in concert with cytoplasmic dynein-1. a motor protein responsible for minus-end-directed microtubule transport. DCTN6. also known as p27. stabilizes the dynactin structure by linking the shoulder/sidearm subcomplex to the actin-related protein (Arp1) filament, ensuring proper cargo binding and motor coordination. This interaction is vital for maintaining cellular functions such as organelle positioning, mitotic spindle organization, and axonal transport in neurons.
Recombinant DCTN6 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for research. Its production typically involves cloning the DCTN6 gene into expression vectors, followed by affinity chromatography for purification. Tags like His or GST are often added to facilitate isolation. Studies using recombinant DCTN6 focus on elucidating dynactin’s role in diseases linked to transport defects, including neurodegenerative disorders (e.g., ALS, Huntington’s) and cancers with aberrant cell division or metastasis. It also serves as a tool to investigate dynein-dynactin-adaptor interactions, cargo recognition mechanisms, and the impact of pathogenic mutations on complex stability. By enabling in vitro reconstitution of transport machinery, recombinant DCTN6 advances therapeutic targeting of microtubule-dependent processes.
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