| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCTN2 |
| Uniprot No | Q13561 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-406aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMADPKYADLPGIARNEPDVYETSDLPE DDQAEFDAFAQELEELTSTSVEHIIVNPNAAYDKFKDKRVGTKGLDFSDR IGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKT TVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGA LAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAA KVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALD LAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPI ASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLT QVQTTMRENLATVEGNFASIDERMKKLGK |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DCTN2重组蛋白的简要参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库检索确认)：
1. **文献名称**：*Dynactin subunit DCTN2 regulates mitotic progression and interphase nuclear shape*
**作者**：Smith A, et al.
**摘要**：研究通过重组DCTN2蛋白的体外功能实验，发现其通过调控动力蛋白复合体(dynactin)的稳定性，影响有丝分裂纺锤体的组装和细胞核形态维持。
2. **文献名称**：*Structural analysis of the DCTN2 subunit in the dynactin complex*
**作者**：Lee C, Zhang Y.
**摘要**：利用重组DCTN2蛋白进行X射线晶体学研究，揭示了其与DCTN1的相互作用界面，阐明了其在维持动力蛋白复合体结构完整性中的关键作用。
3. **文献名称**：*DCTN2-mediated vesicle trafficking in neuronal development*
**作者**：Tanaka K, et al.
**摘要**：通过重组DCTN2蛋白的过表达实验，证明其参与神经元内细胞器运输，并影响轴突生长和突触形成，提示其与神经退行性疾病的潜在关联。
4. **文献名称**：*DCTN2 interacts with HIV-1 capsid protein to promote viral infection*
**作者**：Wang H, et al.
**摘要**：研究发现重组DCTN2蛋白与HIV-1衣壳蛋白直接结合，促进病毒颗粒在宿主细胞内的逆向运输，为抗病毒治疗提供了新靶点。
**提示**：实际研究中建议通过PubMed、Google Scholar等平台检索最新文献，结合关键词"DCTN2 recombinant protein"或"dynactin DCTN2"获取准确信息。
DCTN2 (Dynactin Subunit 2), also known as p50 or dynamitin, is a critical component of the dynactin multiprotein complex, which regulates intracellular transport processes by linking cytoplasmic dynein motors to cargoes. As part of the dynactin complex, DCTN2 plays a pivotal role in maintaining the stability and functionality of the dynein-dynactin motor machinery, enabling retrograde vesicle trafficking, organelle positioning, and mitotic spindle organization. Structurally, it contains a conserved N-terminal domain that facilitates interactions with dynein intermediate chains and other dynactin subunits, such as DCTN1 (p150 glued).
Research highlights DCTN2's involvement in cellular division, neuronal transport, and maintenance of Golgi apparatus integrity. Dysregulation of DCTN2 has been implicated in neurodegenerative disorders (e.g., amyotrophic lateral sclerosis) and cancers due to impaired axonal transport or chromosomal segregation errors. Its role in modulating dynein activity also intersects with viral pathogenesis, as some pathogens exploit this transport system for intracellular replication.
Recombinant DCTN2 proteins are engineered using expression systems (e.g., E. coli, mammalian cells) to preserve post-translational modifications critical for function. These purified proteins serve as essential tools for in vitro studies, including protein interaction assays, structural analysis (e.g., cryo-EM), and drug discovery targeting transport-related diseases. Recent studies leverage recombinant DCTN2 to dissect mechanistic details of dynein regulation and develop therapeutic strategies for transportopathies. Its versatility in research underscores its importance in both basic cell biology and translational medicine.
×
