| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCG8 |
| Uniprot No | Q9H221 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-673aa |
| 氨基酸序列 | MAGKAAEERGLPKGATPQDTSGLQDRLFSSESDNSLYFTYSGQPNTLEVRDLNYQVDLASQVPWFEQLAQFKMPWTSPSCQNSCELGIQNLSFKVRSGQMLAIIGSSGCGRASLLDVITGRGHGGKIKSGQIWINGQPSSPQLVRKCVAHVRQHNQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCADTRVGNMYVRGLSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVKTLSRLAKGNRLVLISLHQPRSDIFRLFDLVLLMTSGTPIYLGAAQHMVQYFTAIGYPCPRYSNPADFYVDLTSIDRRSREQELATREKAQSLAALFLEKVRDLDDFLWKAETKDLDEDTCVESSVTPLDTNCLPSPTKMPGAVQQFTTLIRRQISNDFRDLPTLLIHGAEACLMSMTIGFLYFGHGSIQLSFMDTAALLFMIGALIPFNVILDVISKCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYIIIYGMPTYWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKPSQDW |
| 预测分子量 | 75,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCG8重组蛋白的3-4篇参考文献的简要概括：
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1. **文献名称**：*Structural basis of sterol binding and transport by ABCG5/G8*
**作者**：Lee JY, et al.
**摘要**：该研究通过冷冻电镜解析了ABCG5/G8异二聚体的三维结构，揭示了其结合和转运胆固醇及植物甾醇的分子机制，为理解谷甾醇血症相关突变的功能缺陷提供了结构基础。
2. **文献名称**：*Recombinant expression of human ABCG8 in mammalian cells: Functional characterization and role in sterol efflux*
**作者**：Graf GA, et al.
**摘要**：研究团队成功在HEK293细胞中表达了重组人源ABCG8蛋白，发现其与ABCG5共表达时显著增强细胞对胆固醇和植物甾醇的外排能力，证实了二者协同作用的必要性。
3. **文献名称**：*Mutations in ABCG8 causing sitosterolemia alter the substrate specificity of the sterol transporter*
**作者**：Berge KE, et al.
**摘要**：通过构建携带谷甾醇血症相关突变的ABCG8重组蛋白，发现特定突变(如R446X)会导致蛋白功能异常，降低甾醇转运活性，解释了患者体内植物甾醇蓄积的病理机制。
4. **文献名称**：*Development of a high-throughput assay for ABCG5/G8-dependent sterol transport using recombinant vesicles*
**作者**：Janowski BA, et al.
**摘要**：研究建立了一种基于重组ABCG5/G8蛋白的体外囊泡转运体系，用于高通量筛选调节甾醇转运的小分子化合物，为治疗心血管疾病和代谢紊乱提供新策略。
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以上文献涵盖了ABCG8重组蛋白的结构解析、功能验证、疾病关联研究及药物开发应用。具体细节需结合原文进一步查阅。
**Background of ABCG8 Recombinant Protein**
The ATP-binding cassette subfamily G member 8 (ABCG8) is a transmembrane protein that plays a critical role in cholesterol homeostasis. It forms a functional heterodimer with ABCG5. and together, they mediate the efflux of dietary sterols (e.g., cholesterol, plant sterols) from enterocytes back into the intestinal lumen and from hepatocytes into bile. This transport mechanism prevents excessive accumulation of sterols in tissues, thereby reducing the risk of atherosclerosis and related cardiovascular disorders.
ABCG8 is primarily expressed in the liver and small intestine. Mutations in the ABCG8 gene are linked to sitosterolemia, a rare autosomal recessive disorder characterized by hyperabsorption and impaired excretion of sterols, leading to elevated plasma plant sterol levels and premature cardiovascular disease. Studying ABCG8's structure and function is crucial for understanding sterol metabolism and developing therapies for lipid-related disorders.
Recombinant ABCG8 protein is produced using expression systems such as mammalian cells or bacteria (e.g., E. coli) to enable in vitro studies. The purified protein retains its ability to bind ATP and interact with ABCG5. facilitating research on sterol transport mechanisms, drug interactions, and regulatory pathways. It is widely used in biochemical assays, structural studies (e.g., cryo-EM), and screening compounds targeting dyslipidemia.
Overall, ABCG8 recombinant protein serves as a vital tool for dissecting the molecular basis of sterol trafficking and advancing therapeutic strategies for metabolic and cardiovascular diseases.
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