| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCB8 |
| Uniprot No | Q9NUT2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 38-693aa |
| 氨基酸序列 | GKTGQLHKAEGEYSDGYRSSSLLRAVAHLRSQLWAHLPRAPLAPRWSPSA WCWVGGALLGPMVLSKHPHLCLVALCEAEEAPPASSTPHVVGSRFNWKLF WQFLHPHLLVLGVAVVLALGAALVNVQIPLLLGQLVEVVAKYTRDHVGSF MTESQNLSTHLLILYGVQGLLTFGYLVLLSHVGERMAVDMRRALFSSLLR QDITFFDANKTGQLVSRLTTDVQEFKSSFKLVISQGLRSCTQVAGCLVSL SMLSTRLTLLLMVATPALMGVGTLMGSGLRKLSRQCQEQIARAMGVADEA LGNVRTVRAFAMEQREEERYGAELEACRCRAEELGRGIALFQGLSNIAFN CMVLGTLFIGGSLVAGQQLTGGDLMSFLVASQTVQRSMANLSVLFGQVVR GLSAGARVFEYMALNPCIPLSGGCCVPKEQLRGSVTFQNVCFSYPCRPGF EVLKDFTLTLPPGKIVALVGQSGGGKTTVASLLERFYDPTAGVVMLDGRD LRTLDPSWLRGQVVGFISQEPVLFGTTIMENIRFGKLEASDEEVYTAARE ANAHEFITSFPEGYNTVVGERGTTLSGGQKQRLAIARALIKQPTVLILDE ATSALDAESERVVQEALDRASAGRTVLVIAHRLSTVRGAHCIVVMADGRV WEAGTH |
| 预测分子量 | 87 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCB8重组蛋白的3篇参考文献的简要信息：
---
1. **文献名称**：*ABCB8 mediates doxorubicin resistance in hepatocellular carcinoma by modulating mitochondrial oxidative stress*
**作者**：Chen L, et al.
**摘要**：研究通过重组ABCB8蛋白表达，揭示其在肝癌细胞中对阿霉素耐药的作用机制。发现ABCB8通过调控线粒体氧化应激通路，减少活性氧(ROS)积累，从而增强肿瘤细胞对化疗药物的抵抗。
---
2. **文献名称**：*Structural and functional characterization of recombinant human ABCB8 in lipid nanodiscs*
**作者**：Smith J, et al.
**摘要**：利用重组人源ABCB8蛋白结合脂质纳米盘技术，解析其跨膜结构域和ATP结合位点的功能。实验表明，ABCB8具有ATP依赖性转运活性，可能参与线粒体膜中特定底物的运输。
---
3. **文献名称**：*ABCB8 deficiency alters cardiac mitochondrial lipid composition and induces cardiomyopathy in mice*
**作者**：Wang Y, et al.
**摘要**：通过构建重组ABCB8蛋白模型，研究其在心脏线粒体脂质代谢中的功能。结果表明，ABCB8缺失导致线粒体心磷脂合成异常，引发心肌病，提示其在线粒体膜稳定性中的关键作用。
---
注：以上文献为示例，实际引用需以真实发表的论文为准。建议通过PubMed、Web of Science等平台检索最新研究。
**Background of ABCB8 Recombinant Protein**
ABCB8. a member of the ATP-binding cassette (ABC) transporter superfamily, is a mitochondrial membrane protein implicated in cellular homeostasis and stress responses. ABC transporters are evolutionarily conserved proteins that utilize ATP hydrolysis to transport diverse substrates across biological membranes. ABCB8 localizes primarily to the inner mitochondrial membrane, where it plays a role in mitochondrial iron-sulfur (Fe-S) cluster biogenesis and export, a critical process for electron transport chain function, DNA repair, and antioxidant defense. Dysregulation of ABCB8 has been linked to mitochondrial disorders, cardiovascular diseases, and cancer, underscoring its importance in cellular metabolism and disease pathogenesis.
Recombinant ABCB8 protein is engineered for functional and structural studies to elucidate its molecular mechanisms. Produced via heterologous expression systems (e.g., *E. coli*, mammalian cells*), the recombinant protein retains key domains, including nucleotide-binding domains (NBDs) and transmembrane regions, essential for ATPase activity and substrate transport. Researchers employ it to investigate substrate specificity, ATP-binding kinetics, and interactions with partner proteins like mitoNEET, which regulates Fe-S cluster trafficking. Additionally, ABCB8 recombinant protein aids in drug discovery, enabling high-throughput screening for modulators targeting mitochondrial dysfunction-related diseases. Its study also contributes to understanding chemoresistance in cancers, as ABCB8 may mediate the efflux of chemotherapeutic agents, akin to other ABC transporters like ABCB1 (P-glycoprotein). Despite progress, unresolved questions remain regarding its precise substrates and regulatory networks, driving ongoing research into its physiological and pathological roles.
×
