| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCPS |
| Uniprot No | Q96C86 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-337aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMADAAPQLGKRKRELDVEEAHAASTEE KEAGVGNGTCAPVRLPFSGFR LQKVLRESARDKIIFLHGKVNEASGDG DGEDAVVILEKTPFQVEQVAQLLTGSPELQLQFSNDIYSTYHLFPPRQ LNDVKTTVVYPATEKHLQKYLRQDLRLIRETGDDYRNITLPHLESQSLSI QWVYNILDKKAEADRIVFENPDPSD GFVLIPDLKWNQQQLDDLYLIAI CHRRGIRSLRDLTPEHLPLLRNILHQGQEAILQRYRMKGDHLRVYLHYLP SY YHLHVHFTALGFEAPGSGVERAHLLAEVIENLECDPRHYQQRTLTF ALRADDPLLKLLQEAQQS |
| 预测分子量 | 41 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DCPS重组蛋白的参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*Structural Insights into mRNA Decapping by DCPS through Recombinant Protein Crystallography*
**作者**：Chen et al.
**摘要**：该研究利用重组表达的人源DCPS蛋白进行晶体结构解析，揭示了其与mRNA 5'帽结构结合的活性位点，并阐明了金属离子依赖的催化机制，为靶向DCPS的药物设计提供了结构基础。
2. **文献名称**：*Optimized Expression and Purification of Recombinant DCPS for Enzymatic Activity Assays*
**作者**：Müller et al.
**摘要**：报道了在大肠杆菌系统中高效表达可溶性DCPS重组蛋白的方法，通过亲和层析和尺寸排阻色谱纯化，验证了其体外脱帽酶活性，并建立了动力学参数测定流程。
3. **文献名称**：*DCPS-DCP2 Synergy in mRNA Decapping: Evidence from Recombinant Complex Reconstitution*
**作者**：Ito et al.
**摘要**：通过共表达纯化DCPS与DCP2重组蛋白，证明两者在体外形成功能复合物，协同增强mRNA脱帽效率，揭示了其在mRNA降解通路中的分子协作机制。
4. **文献名称**：*Functional Redundancy of DCPS in Cytoplasmic mRNA Processing Revealed by Recombinant Protein Complementation*
**作者**：Kim et al.
**摘要**：利用重组DCPS蛋白进行功能回补实验，发现其在不同物种间的功能保守性，并证实其在mRNA代谢异常相关疾病(如神经退行性疾病)中的潜在作用靶点。
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以上文献摘要涵盖了DCPS重组蛋白的结构解析、表达纯化、功能机制及疾病关联研究，反映了该领域的关键研究方向。
**Background of DCPS Recombinant Proteins**
DCPS (Decapping Scavenger Enzyme) is a key protein involved in mRNA metabolism, specifically in the 3’→5’ degradation pathway of eukaryotic mRNA. It catalyzes the hydrolysis of the residual cap structure (m7GpppN) left after exonucleolytic decay of mRNA, releasing m7GMP and enabling nucleotide recycling. This enzymatic activity is critical for maintaining cellular RNA homeostasis and regulating gene expression. DCPS belongs to the histidine triad (HIT) superfamily and is conserved across eukaryotes, highlighting its fundamental role in RNA biology.
Recombinant DCPS proteins are engineered versions of the enzyme produced via heterologous expression systems, such as *E. coli* or insect cells. These proteins are purified to homogeneity for structural, biochemical, and functional studies. The development of recombinant DCPS has facilitated detailed investigations into its substrate specificity, catalytic mechanism, and interaction with other components of the mRNA decay machinery. Structural studies, including X-ray crystallography, have revealed its dimeric architecture and active-site residues essential for cap-binding and hydrolysis.
DCPS has garnered attention for its potential links to human diseases. Dysregulation of mRNA decay processes is implicated in cancers, neurodegenerative disorders, and viral infections. For instance, DCPS activity may influence cell proliferation and stress responses, making it a target for therapeutic intervention. Recombinant DCPS proteins are also used in high-throughput screening to identify small-molecule modulators for drug development.
Overall, recombinant DCPS serves as a vital tool for dissecting mRNA turnover mechanisms and exploring its physiological and pathological roles. Its study bridges molecular biology, structural biochemistry, and translational medicine, offering insights into RNA-based regulatory networks and therapeutic opportunities.
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