| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DAP |
| Uniprot No | P51397 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-102aa |
| 氨基酸序列 | SSPPEGKLETKAGHPPAVKAGGMRIVQKHPHTGDTKEEKDKDDQEWESPSPPKPTVFISGVIARGDKDFPPAAAQVAHQKPHASMDKHPSPRTQHIQQPRK |
| 预测分子量 | 38.0kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与DAP重组蛋白相关的文献示例(注：文献为假设性示例，实际引用需核实)：
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1. **文献名称**: *"Expression and Functional Analysis of Recombinant Death-Associated Protein Kinase (DAPK1) in Escherichia coli"*
**作者**: Smith, J. et al.
**摘要**: 研究报道了在大肠杆菌中高效表达具有活性的重组DAP激酶(DAPK1)，并通过体外激酶实验证实其诱导细胞凋亡的功能，为癌症治疗靶点研究提供工具。
2. **文献名称**: *"Purification and Characterization of Recombinant DAP-3: A Mitochondrial Ribosomal Protein with Pro-apoptotic Activity"*
**作者**: Lee, H. & Kim, S.
**摘要**: 利用昆虫杆状病毒系统表达并纯化重组DAP-3蛋白，发现其通过激活线粒体途径促进肿瘤细胞凋亡，揭示了其在癌症中的潜在治疗价值。
3. **文献名称**: *"Structural Insights into Recombinant DAP12 Signaling Module in Innate Immunity"*
**作者**: Zhang, R. et al.
**摘要**: 解析了重组表达的人源DAP12跨膜蛋白与其配体TREM2的复合物晶体结构，阐明了该蛋白在免疫受体信号转导中的分子机制。
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**提示**：若需真实文献，建议在PubMed或Google Scholar以 **"recombinant DAP protein"**、**"DAPK1 expression"** 或 **"DAP12 signaling"** 等关键词检索，并筛选涉及重组表达、功能或结构研究的论文。
DAP (DNAX-activating protein) recombinant protein is a biologically engineered molecule derived from the DAP12 adaptor protein, a key component in immune cell signaling. Originally identified in the 1990s, DAP12 is a transmembrane protein expressed in natural killer (NK) cells, dendritic cells, and myeloid cells. It plays a critical role in activating immune responses by associating with cell surface receptors like NKG2D and TREM (Triggering Receptor Expressed on Myeloid cells). These receptor complexes transmit intracellular signals through immunoreceptor tyrosine-based activation motifs (ITAMs) in DAP12. which recruit kinases like Syk and ZAP70 to initiate downstream signaling cascades.
Recombinant DAP proteins are synthesized using genetic engineering techniques, typically by inserting the DAP12 gene into expression vectors (e.g., E. coli, yeast, or mammalian cell systems) to produce purified proteins. This approach allows scalable production of functional DAP domains for research and therapeutic applications. In immunotherapy, DAP recombinant proteins are explored for modulating immune cell activity, particularly in cancer treatment. For instance, they may enhance NK cell-mediated cytotoxicity or fine-tune T-cell responses in chimeric antigen receptor (CAR) therapies.
Additionally, DAP recombinant proteins serve as tools to study immune receptor interactions, signal transduction mechanisms, and pathological conditions like autoimmune diseases or chronic inflammation. Their stability and controlled production make them advantageous over native proteins extracted from biological sources. Recent studies also investigate engineered DAP variants with modified binding affinities or fusion tags (e.g., Fc regions) to improve therapeutic efficacy or diagnostic utility. As research progresses, DAP recombinant proteins hold promise for advancing precision immunology and next-generation biotherapeutics.
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